Contents: Functional Grade Purified anti-mouse GITR (TNFRSF18)
Catalog Number: 16-5874
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: DTA-1
Isotype: Rat IgG2b
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
|
|
Staining of C57Bl/6 splenocytes with .015 μg of Purified Rat IgG2b Isotype Control (cat. 14-4031) (open histogram) or .015 μg of Purified anti-mouse GITR (DTA-1) (colored histogram) followed by Biotin Anti-Rat IgG (cat. 13-4813)and Streptavidin-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.
|
| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-5874 |
Fluorescein isothiocyanate (FITC) anti-mouse GITR (TNFRSF18, AITR) |
488 |
518 |
FC
|
| 12-5874 |
Phycoerythrin (PE) anti-mouse GITR (TNFRSF18, AITR) |
488 |
575 |
FC
|
| 13-5874 |
Biotin anti-mouse GITR (TNFRSF18, AITR) |
N/A |
N/A |
FC
|
| 14-5874 |
Affinity Purified anti-mouse GITR (TNFRSF18, AITR) |
N/A |
N/A |
FA
FC
IP
|
| 16-5874 |
Functional Grade* Purified anti-mouse GITR (TNFRSF18, AITR) |
N/A |
N/A |
FA
FC
|
| 17-5874 |
Allophycocyanin (APC) anti-mouse GITR (TNFRSF18, AITR) |
633 |
660 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The DTA-1 monoclonal antibody reacts with mouse GITR, Glucocorticoid-Induced TNFR family gene, also known as TNFRSF18. In naïve mice, GITR is expressed predominantly by CD4
+CD25
+ T regulatory cells (Treg) and by CD25
+ CD4
+ CD8
- thymocytes. Stimulation of GITR abrogates Treg cell-mediated suppression. The removal of GITR-expressing Treg cells or the administration of DTA-1 resulted in organ specific autoimmune disease.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This DTA-1 antibody has been reported for use in flow cytometric analysis. It has also been reported for
in vitro and
in vivo blocking of mouse GITR. (Please use Functional Grade purified DTA-1 in functional assays.)
Applications Tested
This DTA-1 antibody has been tested by flow cytometric analysis of mouse splenocyte suspension. This can be used at less than or equal to 0.015 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Shimizu J, Yamazaki S, Takahashi T, Ishida Y, Sakaguchi S. Stimulation of CD25(+)CD4(+) regulatory T cells through GITR breaks immunological self-tolerance. Nat Immunol. 2002 Feb;3(2):135-42.
Related Products
Cat.
16-4031 Functional Grade Purified Rat IgG2b Isotype Control
Mouse Cellular Antigens Chart