Product Information

Contents: Functional Grade Purified anti-human CD244 (2B4, p38)
Catalog Number: 16-5838
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffered saline, pH 7.2, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENT STERILE.
Clone: eBioC1.7 (C1.7)
Isotype: Mouse IgG1, κ

Staining of normal human peripheral blood cells with 0.25 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.25 μg of Purified anti-human CD244 (C1.7) (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5838	Affinity Purified anti-human CD244 (2B4, p38)	N/A	N/A	FC WB
16-5838	Functional Grade* Purified anti-human CD244 (2B4, p38)	N/A	N/A	FA FC WB
13-5838	Biotin anti-human CD244 (2B4, p38)	N/A	N/A	FC
12-5838	Phycoerythrin (PE) anti-human CD244 (2B4, p38)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBioC1.7 monoclonal antibody reacts with human CD244 (2B4, p38). In human, CD244 is a 38 kDa protein expressed on NK cells, a subset of CD8+ T cells, γδ T cells, monocytes, basophils and eosinophils. Binding of the CD244 ligand, CD48, results in NK cell activation, unlike mouse CD244, which is an inhibitory receptor. For CD244 expressed on NK cells, binding of CD48 results in enhanced NK cell cytotoxicity and secretion of IFN-γ. Recently, it was demonstrated that binding of the C1.7 monoclonal antibody and CD48 involve the same residue in the V domain of human CD244, which explains the ability of C1.7 binding to induce activation of NK cells. Binding of C1.7 to CD244 leads to tyrosine phosphorylation and recruitment of the adaptor molecule SAP (SLAM-associated protein). Patients with X-linked lymphoproliferative disorder (XLPD) have a mutation in SAP which renders it unable to bind to phosphorylated CD244.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioC1.7 (C1.7) antibody has been reported for use in flow cytometric analysis, and immunoblotting (WB).

Applications Tested

This eBioC1.7 (C1.7) antibody has been tested by flow cytometric analysis of normal human peripheral blood. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Valiante NM, Trinchieri G. Identification of a novel signal transduction surface molecule on human cytotoxic lymphocytes. J Exp Med. 1993 Oct 1;178(4):1397-406. (C1.7, mAb development, FC, FA, WB, PubMed)

Chuang SS, Kim MH, Johnson LA, Albertsson P, Kitson RP, Nannmark U, Goldfarb RH, Mathew PA. 2B4 stimulation of YT cells induces natural killer cell cytolytic function and invasiveness. Immunology. 2000 Jul;100(3):378-83. (C1.7, FC, FA, PubMed)

Mathew SO, Kumaresan PR, Lee JK, Huynh VT, Mathew PA. Mutational analysis of the human 2B4 (CD244)/CD48 interaction: Lys68 and Glu70 in the V domain of 2B4 are critical for CD48 binding and functional activation of NK cells. J Immunol. 2005 Jul 15;175(2):1005-13. (C1.7, FC, PubMed)

van Emmerik NE, Knoop CJ, Vaessen LM, Balk AH, Mochtar B, Claas FH, Weimar W. C1.7 monoclonal antibody designates high-avidity CD4+ cytotoxic T lymphocytes involved in clinical heart rejection. Transplantation. 1998 Jul 15;66(1):135-8. (C1.7, FC, PubMed)

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