Product Information

Contents: Functional Grade Purified anti-mouse γδTCR
Catalog Number: 16-5811
Sizes: 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: UC7-13D5
Isotype: Armenian Hamster IgG

Two-color surface staining of mouse lymphnodes with anti-mouse gamma delta TCR (UC7-13D5) FITC and anti-mouse CD3e (145-2C11) PE. Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-5811	FITC anti-mouse gamma delta TCR (gd-TCR, gdTCR, TCRgd)	488	518	FC
13-5811	Biotin anti-mouse gamma delta TCR (gd-TCR, gdTCR, TCRgd)	N/A	N/A	FC
14-5811	Affinity Purified anti-mouse gamma delta TCR (gd-TCR, gdTCR, TCRgd)	N/A	N/A	FC IP
16-5811	Functional Grade* Purified anti-mouse gamma delta TCR (gd-TCR, gdTCR, TCRgd)	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

The UC7-13D5 monoclonal antibody reacts with the mouse γδ TCR complex. γδ TCR is expressed by a small subset of T cells in the thymus, peripheral lymphoid tissues, intestinal epithelium, and epidermis. The exact specificity, ligand and function of γδ TCR-bearing T cells are not yet fully understood; it is suggested that these cells recognize bacterial ligands and some tumor cells in the context of MHC class I-like gene products and play a role in regulation of the immune response and during bacterial infection.

Applications Reported

For research use only, not for diagnostic or therapeutic use. UC7-13D5 has been reported for use as a phenotypic marker for γδ TCR-expressing T cells and has been reported for use in flow cytometric analysis, in vitro functional studies and in vivo depletion.

Applications Tested

The UC7-13D5 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions and can be used at less than or equal to 0.5 µg/million cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Taguchi, T., W. K. Aicher, et al. (1991). "Novel function for intestinal intraepithelial lymphocytes. Murine CD3+, gamma/delta TCR+ T cells produce IFN-gamma and IL-5." J Immunol 147(11): 3736-44.
Kakkanaiah, V. N., M. Nagarkatti, et al. (1991). "CD4-CD8- thymocytes from MRL-lpr/lpr mice exhibit abnormal proportions of alpha beta- and gamma delta-TCR+ cells and demonstrate defective responsiveness when activated through the TCR." Cell Immunol 137(2): 269-82.
Bluestone, J. A., R. Q. Cron, et al. (1991). "Repertoire development and ligand specificity of murine TCR gamma delta cells." Immunol Rev 120: 5-33.

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