Contents: Mouse Tim-3-Fc fusion protein
Catalog Number: 16-5771
Formulation: Sterile liquid; 10 mM sodium phosphate, pH 7.2, 150 mM NaCl, with no carrier or stabilizer. 0.22 μM filtered.
Storage Conditions: Store at -80°C.
Avoid repeated freeze/thaw cycles.
Source: Insect cells infected with baculovirus: AQPA-amino acids Leu 22-Arg 192 of mouse TIM-3 (accession # AF450241)-GGRSSLEIEGR-human Fc-His6.
Molecular Mass: The protein has a predicted molecular mass of 49,894. The DTT reduced protein migrates as a band of 58 kDa and the non-reduced protein migrates as a group of bands corresponding to dimer at 110-116 kDa. Heterogeneity results from different glyscosylation states.
Purity: Greater than 98% as determined by SDS-PAGE
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 16-5771 |
Functional Grade* Purified Mouse Tim-3-Fc fusion protein |
N/A |
N/A |
FA
FC
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*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The Tim-3-Fc is a soluble fusion protein derived from expression of a fusion of cDNA encoding the entire extracellular domain of mouse Tim-3 to a cDNA encoding a human IgG1 Fc tail. Tim-3 (T cell immunoglobulin- and mucin domain-containing molecule 3) is a type I membrane protein of 281 amino acids whose extracellular domain consists of an immunoglobulin V-like domain followed by a mucin-like region. A secreted form of Tim-3 containing only the immunoglobulin variable domain of the full length molecule has also been identified. Tim-3 is a Th1-specific cell surface molecule. Tim-3 is polymorphic and, as with other Tim family members, it is linked to asthma. Tim-3-Ig fusion proteins, in which Tim-3 was fused to an Fc tail, have been used in vitro and in vivo to study development of Th1 responses. Based on flow cytometric analysis using full-length and soluble mouse Tim-3 fusion proteins, it has been reported that a putative ligand for Tim-3 is expressed on resting CD4-positive T cells, but not on CD8-positive T cells, B cells, or CD11b-positive leukocytes. Some expression was detected on splenic CD11c-positive dendritic cells. Expression of the ligand was downregulated on activated CD4-positive/CD25-negative effector T cells but persisted on CD4-positive/CD25-positive regulatory T cells. Administration of Tim-3-Ig to immunized mice has been reported to cause hyperproliferation of Th1 cells and amplified Th1 cytokine release, as well as abrogation of tolerance induction in Th1 cells. Interaction of Tim-3 with Tim-3 ligand may serve to inhibit effector Th1 cells during a normal immune response and may be crucial for induction of peripheral tolerance.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The Tim-3-Fc fusion protein has been reported for use in immunofluorescent staining of Tim-3 ligand expressing cells, as well as in vitro and in vivo for induction of Th1 hyperproliferation.
References
Sabatos, A. et al. 2003. Interaction of Tim-3 and Tim-3 ligand regulates T helper type 1 responses and induction of peripheral tolerance. Nature Immunol. 4: 1102-1110.
Sanchez-Fueyo, A., et al. 2003. Tim-3 inhibits T helper type 1-mediated auto- and alloimmune responses and promotes immunological tolerance. Nature Immunol. 4: 1093-1101.
Mouse Cellular Antigens Chart