Product Information
Contents: Functional Grade Purified Mouse IgG2a Isotype Control
Catalog Number: 16-4724
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Isotype: Mouse IgG2a, κ
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-4724 |
Affinity Purified Mouse IgG2a, K Isotype Control |
N/A |
N/A |
FC
|
| 16-4724 |
Functional Grade* Purified Mouse IgG2a, K Isotype Control |
N/A |
N/A |
FC
|
| 13-4724 |
Biotin Mouse IgG2a, K Isotype Control |
N/A |
N/A |
FC
|
| 11-4724 |
Fluorescein isothiocyanate (FITC) Mouse IgG2a, K Isotype Control |
488 |
518 |
FC
|
| 12-4724 |
Phycoerythrin (PE) Mouse IgG2a, K Isotype Control |
488 |
575 |
FC
|
| 15-4724 |
Phycoerythrin-Cy5 (PE-Cy5) Mouse IgG2a, K Isotype Control |
488 |
670 |
FC
|
| 17-4724 |
Allophycocyanin (APC) Mouse IgG2a, K Isotype Control |
633 |
660 |
FC
|
| 25-4724 |
Phycoerythrin-Cy7 (PE-Cy7) Mouse IgG2a, K Isotype Control |
488 |
760 |
FC
|
| 51-4724 |
Alexa Fluor® 647 Mouse IgG2a, K Isotype Control |
633 |
668 |
FC
|
| 53-4724 |
Alexa Fluor® 488 Mouse IgG2a, K Isotype Control |
488 |
519 |
FC
|
| 56-4724 |
Alexa Fluor® 700 Mouse IgG2a, K Isotype Control (APC-Cy5.5 Equivalent) |
633 |
723 |
FC
|
| 57-4724 |
Pacific Blue® Mouse IgG2a, K Isotype Control |
402 |
421 |
FC
|
| 27-4724 |
Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Mouse IgG2a, K Isotype Control (APC-Cy7 Equivalent) |
633 |
779 |
FC
|
| 45-4724 |
PerCP-Cy5.5 Mouse IgG2a, K Isotype Control |
488 |
690 |
FC
|
| 93-4724 |
eFluorTM 605NC Mouse IgG2a, Isotype Control |
350-500 |
605 |
FC
|
| 94-4724 |
eFluorTM 625NC Mouse IgG2a Isotype Control |
350-500 |
625 |
FC
|
| 95-4724 |
eFluorTM 650NC Mouse IgG2a, K Isotype Control |
350-500 |
650 |
FC
|
| 47-4724 |
Allophycocyanin-eFluorTM 780 Mouse IgG2a, K Isotype Control (APC-Alexa Fluor® 750 replacement) |
633 |
780 |
FC
|
| 48-4724 |
eFluorTM 450 Mouse IgG2a, K Isotype Control (Pacific Blue® replacement) |
405 |
450 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
This is a monoclonal mouse IgG2a, κ antibody. It is used as an isotype control for mouse IgG2a antibodies.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This mouse IgG2a isotype control has been reported for use in bioassays and cell surface and intracellular flow cytometric analysis.
Applications Tested
This mouse IgG2a isotype control has been tested by flow cytometric analysis of human peripheral blood leukocytes and can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
