Product Information

Contents: Functional Grade Purified Rat IgG2b Isotype Control
Catalog Number: 16-4331
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Isotype: Rat IgG2b, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
16-4331	Functional Grade* Purified Rat IgG2b Isotype Control	N/A	N/A	FC
10-4331	May be discontinued; see superior product APC-Alexa Fluor® 750 Allophycocyanin-Cy7 (APC-Cy7) Rat IgG2b Isotype Control	633	760	FC
35-4331	Phycoerythrin-Cy5.5 (PE-Cy5.5) Rat IgG2b Isotype Control	488	690	FC
57-4331	DISCONTINUED - Pacific Blue® Rat IgG2b Isotype Control	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

This is a monoclonal rat IgG2b κ. It is used as an isotype control for rat IgG2b antibodies.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This rat IgG2b isotype control has been reported for use in flow cytometric analysis.

Applications Tested

This rat IgG2b isotype control has been used by flow cytometric analysis of mouse splenocyte suspension and can be used at less than or equal to 0.25 μg/million cells. It is recommended that the antibody be carefully titrated for optimal performance in other applications.