Product Information

Contents: Functional Grade Purified Rat IgG2b Isotype Control
Catalog Number: 16-4331
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Isotype: Rat IgG2b, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
57-4331	DISCONTINUED - Pacific Blue® Rat IgG2b Isotype Control	402	421	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

This is a monoclonal rat IgG2b κ. It is used as an isotype control for rat IgG2b antibodies.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This rat IgG2b isotype control has been reported for use in flow cytometric analysis.

Applications Tested

This rat IgG2b isotype control has been used by flow cytometric analysis of mouse splenocyte suspension and can be used at less than or equal to 0.25 μg/million cells. It is recommended that the antibody be carefully titrated for optimal performance in other applications.