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Contents: Functional Grade Purified Rat IgG1 isotype control Catalog Number: 16-4301 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, No NaN3 Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles. KEEP CONTENTS STERILE. Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay. Isotype: Rat IgG1, κ Prices for This Product* Cat. No. Size Price Qty Action 16-4301-81 50 ug $60 16-4301-85 500 ug $180 *International customers: Please contact your distributor for region specific pricing.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
10-4301	**May be discontinued; see superior product APC-Alexa Fluor® 750** Allophycocyanin-Cy7 (APC-Cy7) Rat IgG1 Isotype Control	633	760	FC
11-4301	FITC Rat IgG1 Isotype Control	488	518	FC
12-4301	PE Rat IgG1 Isotype Control	488	575	FC
13-4301	Biotin Rat IgG1 Isotype Control	N/A	N/A	FC
14-4301	Affinity Purified Rat IgG1 Isotype Control	N/A	N/A	FC
15-4301	Phycoerythrin-Cy5 (PE-Cy5) Rat IgG1 Isotype Control	488	670	FC
16-4301	Functional Grade* Purified Rat IgG1 Isotype Control	N/A	N/A	FC
17-4301	APC Rat IgG1 Isotype Control	633	660	FC
19-4301	Cy5 Rat IgG1 Isotype Control	633	670	FC
25-4301	Phycoerythrin-Cy7 (PE-Cy7) Rat IgG1 Isotype Control	488	760	FC
27-4301	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Rat IgG1 Isotype Control (APC-Cy7 Equivalent)	633	779	FC
36-4301	In Vivo Cytokine Capture Assay (IVCCA) In Vivo Capture Antibody – Control / Functional Grade* Biotin Rat IgG1 Isotype Control	N/A	N/A	IVCCA
45-4301	PerCP-Cy5.5 Rat IgG1 Isotype Control	488	690	FC
51-4301	Alexa Fluor® 647 Rat IgG1 Isotype Control	633	668	FC
53-4301	Alexa Fluor® 488 Rat IgG1 Isotype Control	488	519	FC
56-4301	Alexa Fluor® 700 Rat IgG1 Isotype Control (APC-Cy5.5 Equivalent)	633	723	FC
57-4301	Pacific Blue® Rat IgG1 Isotype Control	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.   Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The monoclonal rat IgG1, κ is useful as an isotype control immunoglobulin.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. This rat IgG1 isotype control has been reported for use in flow cytometric analysis.

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Applications Tested

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FG Purified Rat IgG1 Iso Cntrl has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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