Functional Grade Purified anti-human MDR1 (CD243, P-gp, P-glycoprotein, ABCB1)

RUO: For Research Use Only

human CD243 antibody, Functional Grade™ Purified conjugate

Staining of 8226/S (blue histogram) and 8226 DOX40 (purple histogram) cell lines with 0.5 μg FG Purified Mouse IgG2a, K Iso Cntrl (cat. 16-4724) (left) or 0.5 μg FG Purified anti-human MDR1 (CD243, P-gp, P-glycoprotein, ABCB1) (right) followed by PE Donkey F(ab')2 Fragment Anti-Mouse IgG (H+L)(Minimal Reactivity to Rat IgG) (cat. 12-4012). Total viable cells were used for analysis.

Contents: Functional Grade Purified anti-human MDR1 (CD243, P-gp, P-glycoprotein, ABCB1)
Catalog Number: 16-2439
Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Clone: UIC2
Host/Isotype: Mouse IgG2a
Storage Conditions: Store at 2-8°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.

Product Options
Cat. No.	Size	Price	Add Qty to Cart
16-2439-81	50 ug	To view relevant pricing, please select your country and resume browsing.
16-2439-85	500 ug

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
12-4012	Phycoerythrin (PE) Donkey F(ab')2 Fragment Anti-Mouse IgG (H+L)(Minimal Reactivity to Rat IgG) (polyclonal)
16-4724	Functional Grade Purified Mouse IgG2a, K Isotype Control

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
12-2439	Phycoerythrin (PE) anti-human MDR1 (CD243, P-gp, P-glycoprotein, ABCB1)	488	575	FC	RUO
16-2439	Functional Grade* Purified anti-human MDR1 (CD243, P-gp, P-glycoprotein, ABCB1)	N/A	N/A	FA FC	RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Description

This monoclonal antibody reacts with human Multidrug Resistant (MDR)-1, which is also known as P-glycoprotein (Pgp) and CD243. A 170-kDa transmembrane protein, MDR-1 is an ATP-dependent efflux pump for lipophilic compounds, including anti-cancer drugs. Expression of MDR-1 has been shown to correlate with multidrug resistance. In fact, tumor resistance to chemotherapy has been linked to MDR-1 expression in many cancers. MDR-1 is expressed in a variety of tissues, including the brain, kidney, liver, pancreas, and testes. Within the immune system, this molecule can be found on normal T, B, and natural killer cells, but not on monocytes.

This antibody has been reported to inhibit MDR1-mediated efflux.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This UIC2 antibody has been reported for use in functional assays.

Applications Tested

This UIC2 antibody has been pre-titrated and tested by flow cytometric analysis on the multidrug resistant cell line 8226 DOX40 and its parent line 8226/S. This can be used at 1 μg per million cells in a 100 μl total staining volume.

References

Chaudhary PM, Mechetner EB, Roninson IB. Expression and activity of the multidrug resistance P-glycoprotein in human peripheral blood lymphocytes. Blood. 1992 Dec 1;80(11):2735-9. (UIC2, FC)

Mechetner EB, Roninson IB. Efficient inhibition of P-glycoprotein-mediated multidrug resistance with a monoclonal antibody. Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):5824-8. (UIC2, FA)