Also known as: P-gp, P-glycoprotein, Pglycoprotein, P-glycoprotein-1, P-glycoprotein1, P glycoprotein, P glycoprotein-1, P glycoprotein1, ABCB1
RUO: For Research Use Only
Staining of 8226/S (blue histogram) and 8226 DOX40 (purple histogram) cell lines with 0.5 µg Mouse IgG2a K Isotype Control Functional Grade Purified (cat. 16-4724) (left) or 0.5 µg Anti-Human CD243 Functional Grade Purified (right) followed by F(ab')2 Anti-Mouse IgG PE (cat. 12-4012). Total viable cells were used for analysis.
Contents: Anti-Human CD243 (ABCB1) Functional Grade Purified Catalog Number: 16-2439 Concentration: 1 mg/ml Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Storage Conditions: Store at 2-8°C. Handling Conditions: Use in sterile envrioment. Clone: UIC2 Host/Isotype: Mouse IgG2a
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Cat. No.
Size
Price
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16-2439-81
50 ug
16-2439-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
This monoclonal antibody reacts with human Multidrug Resistant (MDR)-1, which is also known as P-glycoprotein (Pgp) and CD243. A 170-kDa transmembrane protein, MDR-1 is an ATP-dependent efflux pump for lipophilic compounds, including anti-cancer drugs. Expression of MDR-1 has been shown to correlate with multidrug resistance. In fact, tumor resistance to chemotherapy has been linked to MDR-1 expression in many cancers. MDR-1 is expressed in a variety of tissues, including the brain, kidney, liver, pancreas, and testes. Within the immune system, this molecule can be found on normal T, B, and natural killer cells, but not on monocytes.
This antibody has been reported to inhibit MDR1-mediated efflux.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This UIC2 antibody has been reported for use in functional assays.
Applications Tested
This UIC2 antibody has been pre-titrated and tested by flow cytometric analysis on the multidrug resistant cell line 8226 DOX40 and its parent line 8226/S. This can be used at 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
References
Chaudhary PM, Mechetner EB, Roninson IB. Expression and activity of the multidrug resistance P-glycoprotein in human peripheral blood lymphocytes. Blood. 1992 Dec 1;80(11):2735-9. (UIC2, FC)
Mechetner EB, Roninson IB. Efficient inhibition of P-glycoprotein-mediated multidrug resistance with a monoclonal antibody. Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):5824-8. (UIC2, FA)
