Staining of C57Bl/6 splenocytes with 0.5 μg of Purified Armenian Hamster IgG Iso Cntrl (cat. 14-4888) (open histogram) or 0.5 μg of Purified anti-mouse SIGN-R1 (eBio22D1) (colored histogram) followed by PE anti-Armenian Hamster IgG (cat. 12-4112). Cells in the lymphocyte and monocyte gates were used for analysis, and events displayed are gated on CD11c+ cells.
Contents: Functional Grade Purified anti-mouse SIGN-R1 (C-type lectin, CD209b) Catalog Number: 16-2093 Formulation: Phosphate buffered saline, pH 7.2, No NaN3 Clone: eBio22D1 (22D1) Host/Isotype: Armenian Hamster IgG Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles. KEEP CONTENT STERILE.
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16-2093-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The eBio22D1 monoclonal antibody reacts with mouse SIGNR1 (CD209b). SIGNR1 is a type II transmembrane C-type lectin that was identified in a search for mouse homologues of human DC-SIGN. It is expressed at high levels in splenic marginal zone macrophages and lymph node medullary macrophages, where it functions to uptake dextran polysaccharides, including the capsular polysaccharide of Streptococcus pneumoniae. It has also been demonstrated that SIGNR1 physically associates with TLR4/MD2, and it has been suggested that this association plays a role in recognition of LPS. Furthermore, recently it has been shown that SIGNR1 deficient mice have a defect in catabolism of the complement component C3, and that SIGNR1 binds directly to the complement C1 subcomponent, C1q to assemble a non-conventional C3 convertase. The eBio22D1 monoclonal antibody does not cross-react with the closely related SIGNR1, SIGNR2, SIGNR3 or SIGNR4.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBio22D1 (22D1) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunohistology staining of frozen tissue sections, and ELISA. in vivo injection of 22D1 has been shown to induce temporary knockdown of SIGNR1 expression.
Applications Tested
This eBio22D1 (22D1) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Kang YS, Kim JY, Bruening SA, Pack M, Charalambous A, Pritsker A, Moran TM, Loeffler JM, Steinman RM, Park CG.
The C-type lectin SIGN-R1 mediates uptake of the capsular polysaccharide of Streptococcus pneumoniae in the marginal zone of mouse spleen.
Proc Natl Acad Sci U S A. 2004 Jan 6;101(1):215-20. (22D1, FC, FA, IHC, PubMed)
Kang YS, Do Y, Lee HK, Park SH, Cheong C, Lynch RM, Loeffler JM, Steinman RM, Park CG.
A dominant complement fixation pathway for pneumococcal polysaccharides initiated by SIGN-R1 interacting with C1q.
Cell. 2006 Apr 7;125(1):47-58. (22D1, IHC frozen, FA, PubMed)
Kang YS, Yamazaki S, Iyoda T, Pack M, Bruening SA, Kim JY, Takahara K, Inaba K, Steinman RM, Park CG.
SIGN-R1, a novel C-type lectin expressed by marginal zone macrophages in spleen, mediates uptake of the polysaccharide dextran.
Int Immunol. 2003 Feb;15(2):177-86.
Nagaoka K, Takahara K, Tanaka K, Yoshida H, Steinman RM, Saitoh S, Akashi-Takamura S, Miyake K, Kang YS, Park CG, Inaba K.
Association of SIGNR1 with TLR4-MD-2 enhances signal transduction by recognition of LPS in gram-negative bacteria.
Int Immunol. 2005 Jul;17(7):827-36.
