Product Information

Contents: Functional Grade Purified anti-mouse CD201 (EPCR, endothelial protein C receptor)
Catalog Number: 16-2012
Sizes: 50 ug, 200 ug
Formulation: Phosphate buffered saline, pH 7.2, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENT STERILE.
Clone: eBio1560 (1560)
Isotype: Rat IgG2b, κ

Staining of bEnd.3 cells with 0.25 μg of Purified Rat IgG2b Isotype Control (cat. 14-4031) (open histogram) or 0.06 μg of Purified anti-mouse CD201/EPCR (1560) (colored histogram) followed by PE Anti-Rat IgG (cat. 12-4822). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-2012	Affinity Purified anti-mouse CD201 (EPCR, endothelial protein C receptor)	N/A	N/A	FA FC IHC
16-2012	Functional Grade* Purified anti-mouse CD201 (EPCR, endothelial protein C receptor)	N/A	N/A	FA FC IHC
13-2012	Biotin anti-mouse CD201 (EPCR, endothelial protein C receptor)	N/A	N/A	FC IHC
12-2012	Phycoerythrin (PE) anti-mouse CD201 (EPCR, endothelial protein C receptor)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBio1560 monoclonal antibody reacts with mouse Endothelial Protein C Receptor (EPCR, CD201), a 25 kDa Type 1 transmembrane protein expressed on endothelial cells. EPCR exhibits sequence and structural homology with the MHC class I/CD1 family of proteins. EPCR is a ligand for Protein C and plays an important role in augmenting Protein C activation by the thrombin-thrombomodulin complex and in regulating blood coagulation and inflammation. Deletion of EPCR results in embryonic lethality, at least partically due to placental thrombosis.

Recently, it was demonstrated that EPCR expression identified cells in the bone marrow that are capable of hematopoietic reconstitution activity comparable to hematopoietic stem cells isolated with conventional methods. The eBio1560 monoclonal antibody can be used for the detection of these hematopoietic stem cells, however the eBiomRCR-16 monoclonal antibody should only be used for the detection of CD201 on endothelial cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio1560 (1560) antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. The eBio1560 monoclonal antibody has been reported to block protein C activation by thrombin on cells expressing both thrombomodulin and CD201.

Applications Tested

This eBio1560 (1560) antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest, particularly when analyzing expression on endothelial cells where CD201 expression is very high.

References

Balazs AB, Fabian AJ, Esmon CT, Mulligan RC. Endothelial protein C receptor (CD201) explicitly identifies hematopoietic stem cells in murine bone marrow. Blood. 2006 Mar 15;107(6):2317-21. Epub 2005 Nov 22. (1560, FC, FA, PubMed)

Fukudome K, Esmon CT. Molecular cloning and expression of murine and bovine endothelial cell protein C/activated protein C receptor (EPCR). The structural and functional conservation in human, bovine, and murine EPCR. J Biol Chem. 1995 Mar 10;270(10):5571-7.

Li W, Zheng X, Gu JM, Ferrell GL, Brady M, Esmon NL, Esmon CT. Extraembryonic expression of EPCR is essential for embryonic viability. Blood. 2005 Oct 15;106(8):2716-22.

Related Products

Cat. 16-4301 Functional Grade Purified Rat IgG1 Isotype Control