Product Information

Contents: Functional Grade Purified anti-human CD154 (CD40 Ligand, CD40L)
Catalog Number: 16-1548
Sizes: 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: 24-31
Isotype: Mouse IgG1, κ
HLDA No.: N/A

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1548	Affinity Purified anti-human CD154 (CD40 Ligand, CD40L)	N/A	N/A	FA FC
16-1548	Functional Grade* Purified anti-human CD154 (CD40 Ligand, CD40L)	N/A	N/A	FA FC
11-1548	FITC anti-human CD154 (CD40 Ligand, CD40L)	488	518	FC
12-1548	PE anti-human CD154 (CD40 Ligand, CD40L)	488	575	FC
17-1548	Allophycocyanin (APC) anti-human CD154 (CD40 Ligand, CD40L)	633	660	FC
47-1548	Allophycocyanin-eFluor^TM 780 anti-human CD154 (CD40 Ligand, CD40L)	633	780	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 24-31 monoclonal antibody reacts with human CD154, a 39 kDa transmembrane glycoprotein also known as gp39 and CD40 ligand (CD40L). CD154 is a member of the TNF superfamily and is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells including B cells, monocytes/macrophages and dendritic cells, CD154 serves a crucial function in T-APC cognate interaction. CD154 interaction with CD40 transduces signals for T-dependent B-cell activation and induces B cell cycle entry. 24-31 cross-reacts with the Cynomogus monkey CD154.

Applications Reported

For research use only, not for diagnostic or therapeutic use. 24-31 has been reported for use in flow cytometric analysis. 24-31 has also been reported to block CD154 binding to CD40 and inhibit T cell-dependent B cell differentiation and antibody response in vitro.

Applications Tested

The 24-31 antibody has been tested by flow cytometric analysis of activated human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Brams, P., A. Black, et al. 2001. A humanized anti-human CD154 monoclonal antibody blocks CD154-CD40 mediated human B cell activation. Int Immunopharmacol 1(2): 277-94.

Related Products

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