Product Information

Contents: Functional Grade Purified anti-mouse CD153 (CD30 Ligand, CD30L)
Catalog Number: 16-1531
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: RM153
Isotype: Rat IgG2b, κ

Surface staining of ConA stimulated mouse splenocytes with anti-mouse CD153 (RM153) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1531	Affinity Purified anti-mouse CD153 (CD30 Ligand, CD30L)	N/A	N/A	FA FC IH/F IP
16-1531	Functional Grade* Purified anti-mouse CD153 (CD30 Ligand, CD30L)	N/A	N/A	FA FC
13-1531	Biotin anti-mouse CD153 (CD30 Ligand, CD30L)	N/A	N/A	FC
12-1531	PE anti-mouse CD153 (CD30 Ligand, CD30L)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The RM153 monoclonal antibody reacts with mouse CD153 (CD30 ligand). This 40 kDa cell surface receptor, a type II transmembrane protein, is a member of the TNF superfamily. Interaction of CD30 with CD153 plays an important role in T cell activation/costimulation. Expression of CD153 on T cells does not appear to require costimulation through CD28 and is upregulated via TCR engagement and peaks 24-48 hours after activation. Signaling through CD153 is important in costimulation of pre-activated T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The RM153 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of ligand binding.

Applications Tested

The RM153 antibody has been tested by flow cytometric analysis of activated mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Shimozato, O., K. Takeda, et al. (1999). "Expression of CD30 ligand (CD153) on murine activated T cells." Biochem Biophys Res Commun 256(3): 519-26.

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