Product Information

Contents: Functional Grade Purified anti-human CD150 (SLAM, IPO-3)
Catalog Number: 16-1509
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: A12 (7D4)
Isotype: Mouse IgG1, κ
HLDA No.: N/A

Surface staining of normal human peripheral blood cells with anti-human CD150 (A12) PE. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1509	Affinity Purified anti-human CD150 (SLAM, IPO-3, IPO3)	N/A	N/A	FA FC IHC
16-1509	Functional Grade* Purified anti-human CD150 (SLAM, IPO-3, IPO3)	N/A	N/A	FA FC
13-1509	Biotin anti-human CD150 (SLAM, IPO-3, IPO3)	N/A	N/A	FC
11-1509	FITC anti-human CD150 (SLAM, IPO-3, IPO3)	488	518	FC
12-1509	PE anti-human CD150 (SLAM, IPO-3, IPO3)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

The A12 monoclonal antibody reacts with human CD150, an ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM). CD150 is expressed by T and B cells and its expression is rapidly upregulated on these cells upon activation. Immature thymocytes and dendritic cells also express this antigen. Signaling through SLAM induces proliferation and cytokine production of T cells and SLAM plays a role in activation of T and B cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. A12 (7D4) has been reported for use in flow cytometric analysis. A12 has also been reported in in vitro functional studies.

Applications Tested

The A12 (7D4) antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Cocks, B. G., C. C. Chang, et al. (1995). "A novel receptor involved in T-cell activation." Nature 376(6537): 260-3.

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