Product Information

Contents: Functional Grade Purified anti-mouse CD150 (SLAM, IPO-3)
Catalog Number: 16-1501
Sizes: 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: 9D1
Isotype: Rat IgG1

Staining of C57Bl/6 splenocytes with .5 μg of Purified Rat IgG1 Iso Cntrl (cat. 14-4301) (open histogram) or .5 μg of Purified anti-mouse CD150 (9D1) (colored histogram) followed by Biotin Anti-Rat IgG (cat. 13-4813)and Streptavidin-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1501	Affinity Purified anti-mouse CD150 (SLAM, IPO-3)	N/A	N/A	FA FC
16-1501	Functional Grade* Purified anti-mouse CD150 (SLAM, IPO-3)	N/A	N/A	FA FC
13-1501	Biotin anti-mouse CD150 (SLAM, IPO-3)	N/A	N/A	FC
11-1501	Fluorescein isothiocyanate (FITC) anti-mouse CD150 (SLAM, IPO-3)	488	518	FC
12-1501	Phycoerythrin (PE) anti-mouse CD150 (SLAM, IPO-3)	488	575	FC
17-1501	Allophycocyanin (APC) anti-mouse CD150 (SLAM, IPO-3)	633	660	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 9D1 monoclonal antibody reacts with mouse CD150, an ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM). CD150 is expressed by T (especially TH1) and B cells and its expression is rapidly upregulated on these cells upon activation. Immature thymocytes and dendritic cells also express this antigen. Signaling through SLAM in T cells induces proliferation and augmentation of the interferon-gamma response. Furthermore, SLAM is thought to play a role in adhesion between the T cell and antigen-presenting cell. 9D1 is reported to be an activating antibody.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 9D1 antibody has been reported for use in flow cytometric analysis. 9D1 has also been reported to augment T-cell activation including inducing their proliferation and production of IFN-γ.

Applications Tested

This 9D1 antibody has been tested by flow cytometric analysis of mouse thymic and splenic cell suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Howie D, Okamoto S, Rietdijk S, Clarke K, Wang N, Gullo C, Bruggeman JP, Manning S, Coyle AJ, Greenfield E, Kuchroo V, Terhorst C. 2002. The role of SAP in murine CD150 (SLAM)-mediated T-cell proliferation and interferon gamma production. Blood. 100(8): 2899-907. (9D1, FC, FA, PubMed)

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Cat. 16-4301 Functional Grade Purified Rat IgG1 Isotype Control