Staining of NIH-3T3 cells with 0.5 μg of FG Purified Rat IgG2a Iso Cntrl (cat. 16-4321) (open histogram) or 0.5 μg FG Purified anti-mouse CD140b (APB5) (colored histogram) followed by Biotin Anti-Rat IgG (cat. 13-4813) and SAv-PE (cat. 12-4317). Total viable cells were used for analysis.
Contents: Functional Grade Purified anti-mouse CD140b (PDGFRβ) Catalog Number: 16-1402 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, No NaN3 Clone: APB5 Host/Isotype: Rat IgG2a, κ Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles. KEEP CONTENTS STERILE. Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
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16-1402-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The APB5 monoclonal antibody reacts with the mouse CD140b molecule, the β chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRb is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of α/α, β/β, or heterodimers of α/β and serve as a substrate for its kinase activity. CD140b is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues. The PDGFR β chain is reported to play a significant role in formation of fibrous atherosclerotic lesions.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The APB5 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of ligand binding.
Applications Tested
The APB5 antibody has been tested by flow cytometric analysis of NIH-3T3 cells. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Sano, H., T. Sudo, et al. 2001. Functional blockade of platelet-derived growth factor receptor-beta but not of receptor-alpha prevents vascular smooth muscle cell accumulation in fibrous cap lesions in apolipoprotein E-deficient mice. Circulation 103(24): 2955-60.
Takakura, N., H. Yoshida, et al. 1997. PDGFR alpha expression during mouse embryogenesis: immunolocalization analyzed by whole-mount immunohistostaining using the monoclonal anti-mouse PDGFR alpha antibody APA5. J Histochem Cytochem 45(6): 883-93.
Sano H, Yokode M, Takakura N, Takemura G, Doi T, Kataoka H, Sudo T, Nishikawa S, Fujiwara H, Nishikawa SI,and Kita S. (2001) Study on PDGF Receptor ß Pathway in Glomerular Formation in Neonate Mice Annals of the New York Academy of Sciences 947:303-305 (FA)
