Product Information

Contents: Functional Grade Purified anti-mouse CD140a (PDGFRa)
Catalog Number: 16-1401
Sizes: 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENT STERILE.
Clone: APA5
Isotype: Rat IgG2a, κ

Staining of NIH/3T3 cells with 0.5 μg of Purified Rat IgG2a Iso Cntrl (cat. 14-4321) (open histogram) or 0.5 μg of Purified anti-mouse CD140a (APA5) (colored histogram) followed by Biotin Anti-Rat IgG (cat. 13-4813)and SAv-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1401	Affinity Purified anti-mouse CD140a (PDGFRa)	N/A	N/A	FC IH/F WB
16-1401	Functional Grade* Purified anti-mouse CD140a (PDGFRa)	N/A	N/A	FA FC IH/F WB
13-1401	Biotin anti-mouse CD140a (PDGFRa)	N/A	N/A	FC
12-1401	Phycoerythrin (PE) anti-mouse CD140a (PDGFRa)	488	575	FC
17-1401	Allophycocyanin (APC) anti-mouse CD140a (PDGFRa)	633	660	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the α chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRa is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of α/α, β/β, or heterodimers of α/β and serve as a substrate for its kinase activity. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This APA5 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistology staining of frozen tissue sections. It has also been reported for blocking ligand binding.

Applications Tested

This APA5 antibody has been tested by flow cytometric analysis of the NIH-3T3 cell line. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Takakura, N., H. Yoshida, et al. (1996). "Involvement of platelet-derived growth factor receptor-alpha in hair canal formation [comment]." J Invest Dermatol 107(5): 770-7.
Takakura, N., H. Yoshida, et al. (1997). "PDGFR alpha expression during mouse embryogenesis: immunolocalization analyzed by whole-mount immunohistostaining using the monoclonal anti-mouse PDGFR alpha antibody APA5." J Histochem Cytochem 45(6): 883-93.

Related Products

Cat. 16-4321 Functional Grade Purified Rat IgG2a Isotype Control