Product Information

Contents: Functional Grade Purified anti-mouse CD122 (Interleukin-2 Receptor beta, IL-2 Receptor beta, IL-2Rb)
Catalog Number: 16-1222
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: TM-b1 (TM-beta1)
Isotype: Rat IgG2b

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1222	Purified anti-mouse CD122 (Interleukin-2 Receptor beta, IL-2 Receptor beta, IL-2Rb, IL2Rb)	N/A	N/A	FC IP NU
16-1222	Functional Grade* Purified anti-mouse CD122 (Interleukin-2 Receptor beta, IL-2 Receptor beta, IL-2Rb, IL2Rb)	N/A	N/A	FA FC
13-1222	Biotin anti-mouse CD122 (Interleukin-2 Receptor beta, IL-2 Receptor beta, IL-2Rb, IL2Rb)	N/A	N/A	FC
11-1222	Fluorescein isothiocyanate (FITC) anti-mouse CD122 (Interleukin-2 Receptor beta, IL-2 Receptor beta, IL-2Rb, IL2Rb)	488	518	FC
12-1222	Phycoerythrin (PE) anti-mouse CD122 (Interleukin-2 Receptor beta, IL-2 Receptor beta, IL-2Rb, IL2Rb)	488	575	FC
57-1222	Pacific Blue® anti-mouse CD122 (Interleukin-2 Receptor beta, IL-2 Receptor beta, IL-2Rb)	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The TM-β1 monoclonal antibody reacts with mouse CD122, the 90-110 kDa interleukin-2 receptor β chain (IL-2Rβ). CD122 is expressed by NK cells and some T cells in the periphery, and is upregulated by activation. CD122 associates with CD25 and CD132 (common γ chain) to form the high affinity IL-2 receptor. High affinity binding of IL-2 to the IL-2 receptor is inhibited by the TM-β1 antibody.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This TM-b1 (TM-beta1) antibody has been reported for use in flow cytometric analysis. It has also been reported to block high affinity binding of IL-2 to its receptor.

Applications Tested

The TM-b1 (TM-beta1) antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Takeuchi Y, Tanaka T, Hamamura K, Sugimoto T, Miyasaka M, Yagita H, Okumura K. 1992. Expression and role of interleukin-2 receptor beta chain on CD4-CD8- T cell receptor alpha beta+ cells. Eur J Immunol. 22(11):2929-35.

Tanaka T, Takeuchi Y, Shiohara T, Kitamura F, Nagasaka Y, Hamamura K, Yagita H, Miyasaka M. 1992. In utero treatment with monoclonal antibody to IL-2 receptor beta-chain completely abrogates development of Thy-1+ dendritic epidermal cells. Int Immunol. 4(4):487-91.

French JD, Roark CL, Born WK, O'brien RL. {gamma}{delta} T cell homeostasis is established in competition with {alpha}{beta} T cells and NK cells. Proc Natl Acad Sci U S A. 2005 Oct 11;102(41):14741-6. Epub 2005 Oct 3. (blocking, PubMed)

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Cat. 16-4031    Functional Grade Purified Rat IgG2b Isotype Control