Functional Grade Purified anti-mouse CD117 (c-Kit, cKit) - neutralizing

RUO: For Research Use Only

Contents: Functional Grade Purified anti-mouse CD117 (c-Kit, cKit) - neutralizing
Catalog Number: 16-1172
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Clone: ACK2
Host/Isotype: Rat IgG2b, κ
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.

Product Options
Cat. No.	Size	Price	Add Qty to Cart
16-1172-81	50 ug	To view relevant pricing, please select your country and resume browsing.
16-1172-82	100 ug
16-1172-85	500 ug

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
11-4317	Streptavidin-FITC (Fluorescein isothiocyanate)
11-4811	FITC Anti-Rat IgG
12-4317	Streptavidin-PE (Phycoerythrin)
13-4813	Biotin Anti-Rat IgG (Polyclonal)
16-4031	Functional Grade Purified Rat IgG2b Isotype Control
17-4317	Streptavidin Allophycocyanin (SA-APC)

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
12-1172	PE anti-mouse CD117 (c-Kit, cKit)	488	575	FC	RUO
14-1172	Affinity Purified anti-mouse CD117 (c-Kit, cKit) - neutralizing	N/A	N/A	FA FC IH/F IP WB	RUO
15-1172	PE-Cy5 anti-mouse CD117 (c-Kit, cKit)	488	670	FC	RUO
16-1172	Functional Grade* Purified anti-mouse CD117 (c-Kit, cKit) - neutralizing	N/A	N/A	FA FC	RUO
17-1172	APC anti-mouse CD117 (c-Kit, cKit)	633	660	FC	RUO
25-1172	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD117 (c-Kit, cKit)	488	760	FC	RUO
47-1172	Allophycocyanin-eFluor® 780 anti-mouse CD117 (c-Kit, cKit) - neutralizing (APC-Alexa Fluor® 750 replacement)	633	780	FC	RUO
56-1172	Alexa Fluor® 700 anti-mouse CD117 (c-Kit, cKit) (APC-Cy5.5 Equivalent)	633	723	FC	RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Description

The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1^lo) and negative for lineage markers. The interaction of the mouse c-kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells. ACK2 has been reported to be a blocking antibody.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The ACK2 antibody has been reported for use in flow cytometric analysis. It has also been reported for use in functional assays.

Applications Tested

The ACK2 antibody has been tested by flow cytometric analysis of mouse bone marrow cell suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Rico-Vargas, S., et al. 1994. c-kit expresssion by B cell precursors in mouse bone marrow. J. Immunol. 152: 2845.

Ito M, Kawa Y, et al. 1999. Removal of stem cell factor or addition of monoclonal anti-c-KIT antibody induces apoptosis in murine melanocyte precursors. J Invest Dermatol. 112: 796-801.

Vincent S, Segretain D, et al. 1998. Stage-specific expression of the Kit receptor and its ligand (KL) during male gametogenesis in the mouse: a Kit-KL interaction critical for meiosis. Development. 125: 4585-93.

Sato D, Lai ZF, et al. 1996. Impairment of Kit-dependent development of interstitial cells alters contractile responses of murine intestinal tract. Am J Physiol. 271: G762-71.

Yoshida H, Kunisada T, et al. 1996. Distinct stages of melanocyte differentiation revealed by anlaysis of nonuniform pigmentation patterns. Development. 122: 1207-14.

Yoshinaga K, Nishikawa S, et al. 1991. Role of c-kit in mouse spermatogenesis: identification of spermatogonia as a specific site of c-kit expression and function. Development. 113:689-99.

Torihashi S, Ward SM, et al. 1995. c-kit-dependent development of interstitial cells and electrical activity in the murine gastrointestinal tract. Cell Tissue Res. 280:97-111.

Feng H, Sandlow JI, Sandra A. 1997. Expression and function of the c-kit proto-oncogene protein in mouse sperm. Biol Reprod. 57:194-203.

Feng H, Sandlow JI, Sandra A. 1998. The c-kit receptor and its possible signaling transduction pathway in mouse spermatozoa. Mol Reprod Dev. 49:317-26.

Prasanth SG and Ali S. 2003. Expression of proto-oncogene c-kit receptor in rats (Rattus norvegicus) and identification of a mutant mRNA transcript implicated in spermatogenic failure. DNA Cell Biol. 2003 Jul;22(7):447-56. (ACK2, rat cross-reactivity, PubMed)

Ismail RS, Okawara Y, et al. 1996. Hormonal regulation of the ligand for c-kit in the rat ovary and its effects on spontaneous oocyte meiotic maturation. Mol Reprod Dev. 1996 Apr;43(4):458-69. (ACK2, rat cross-reactivity and blocking, PubMed)

Kim HM, Shin HY, and Lee EH. 1998. Morphological alterations in rat peritoneal mast cells by stem cell factor. Immunology. 1998 Jun;94(2):242-6. (ACK2, rat cross-reactivity and blocking, PubMed)

Czechowicz A, Kraft D, Weissman IL, and Bhattacharya D. 2007. Efficient transplantation via antibody-based clearance of hematopoietic stem cell niches. Science. 23;318(5854):1296-9. (ACK2, in vivo depletion HSC, PubMed)