Product Information

Contents: Functional Grade Purified anti-mouse CD115 (FMS, CSF-1R)
Catalog Number: 16-1152
Sizes: 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: AFS98
Isotype: Rat IgG2a, κ

Surface staining of thioglycolate induced PEC with anti-mouse CD115 (AFS98) PE. Appropriate isotype controls were used (open histogram). Cells in the large scatter population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1152	Affinity Purified anti-mouse CD115 (FMS, CSF-1R, CSF-1 receptor)	N/A	N/A	FA FC IH/F WB
16-1152	Functional Grade* Purified anti-mouse CD115 (FMS, CSF-1R, CSF-1 receptor)	N/A	N/A	FA FC
13-1152	Biotin anti-mouse CD115 (FMS, CSF-1R, CSF-1 receptor)	N/A	N/A	FC
12-1152	PE anti-mouse CD115 (FMS, CSF-1R, CSF-1 receptor)	488	575	FC
17-1152	Allophycocyanin (APC) anti-mouse CD115 (FMS, CSF-1R, CSF-1 receptor)	633	660	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells. It is a 150 kDa c-fms gene product and belongs to immunoglobulin family. CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The AFS98 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of ligand binding.

Applications Tested

The AFS98 antibody has been tested by blocking of fluorochrome conjugated AFS98 in flow cytometric analysis of peritoneal exudate cells. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Murayama T, Yokode M, et al. 1999. Intraperitoneal administration of anti-c-fms monoclonal antibody prevents initial events of atherogenesis but does not reduce the size of advanced lesions in apolipoprotein E-deficient mice. Circulation. 99(13): 1740-6.

Sudo T, Nishikawa S, et al. 1995. Functional hierarchy of c-kit and c-fms in intramarrow production of CFU-M. Oncogene. 11(12): 2469-76.

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