Product Information

Contents: Functional Grade Purified anti-rat CD106 (VCAM-1)
Catalog Number: 16-1060
Sizes: 500 ug
Formulation: Phosphate buffered saline, pH 7.2, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENT STERILE.
Endotoxin Level: Less than 0.01 ng/ug antibody, as determined by the LAL assay.
Clone: eBioMR106 (MR106)
Isotype: Mouse IgG1, κ

Staining of Lou rat bone marrow cells with 0.25 µg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.25 µg of FG Purified anti-rat CD106 (MR106) (colored histogram) followed by Biotin Anti-Mouse IgG (cat. 13-4013)and Streptavidin-PE (cat. 12-4317). Cells in the large scatter population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1060	Affinity Purified anti-rat CD106 (VCAM-1)	N/A	N/A	FC IH/F IP WB
16-1060	Functional Grade* Purified anti-rat CD106 (VCAM-1)	N/A	N/A	FC
13-1060	Biotin anti-rat CD106 (VCAM-1)	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBioMR106 monoclonal antibody reacts with rat CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and further upregulate VCAM-1 upon cytokine stimulation. CD106 binds to integrin α4β1 (VLA-4, CD49d/CD29) and Integrin α4β7 (LPAM-1) and these interactions in the bone marrow and thymus are important for early lymphocyte and myeloid development. Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioMR106 (MR106) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioMR106 (MR106) antibody has been tested by flow cytometric analysis of rat bone marrow cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Williams AJ, Atkins RC, Fries JW, Gimbrone MA Jr, Cybulsky MI, Collins T. Nucleotide sequence of rat vascular cell adhesion molecule-1 cDNA. Biochim Biophys Acta. 1992 Jun 15;1131(2):214-6.

Related Products

Cat. 16-1061    Functional Grade Purified anti-mouse CD106 (VCAM-1) (clone 429)
Cat. 11-4011    FITC Anti-Mouse IgG
Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 16-4714    Functional Grade Purified Mouse IgG1, K Isotype Control