Functional Grade Purified anti-human CD95 (Fas/APO-1)

RUO: For Research Use Only

Contents: Functional Grade Purified anti-human CD95 (Fas/APO-1)
Catalog Number: 16-0958
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, No NaN₃
Clone: EOS9.1
Host/Isotype: Mouse IgM, κ
HLDA Workshop: N/A
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.01 ng/ug antibody, as determined by the LAL assay.

Product Options
Cat. No.	Size	Price	Add Qty to Cart
16-0958-81	50 ug	To view relevant pricing, please select your country and resume browsing.
16-0958-85	500 ug

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
11-0959	FITC anti-human CD95 (Fas/APO-1) (DX2)
11-4317	Streptavidin-FITC (Fluorescein isothiocyanate)
11-5790	Fluorescein (FITC) anti-mouse IgM (II/41)
12-0959	PE anti-human CD95 (Fas/APO-1) (DX2)
12-4317	Streptavidin-PE (Phycoerythrin)
13-0959	Biotin anti-human CD95 (Fas/APO-1) (DX2)
13-5790	Biotin anti-mouse IgM (II/41)
14-0959	Affinity Purified anti-human CD95 (Fas/APO-1) (DX2)
15-0959	PE-Cy5 anti-human CD95 (Fas/APO-1) (DX2)
17-4317	Streptavidin Allophycocyanin (SA-APC)

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
14-0958	Affinity Purified anti-human CD95 (Fas/APO-1)	N/A	N/A	FA FC	RUO
16-0958	Functional Grade* Purified anti-human CD95 (Fas/APO-1)	N/A	N/A	FA FC	RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Description

The EOS9.1 monoclonal antibody reacts with human CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, activated lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. EOS9.1 does not block binding of DX2, another antibody specific for human CD95.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The EOS9.1 antibody has been reported for use in flow cytometric analysis. EOS9.1 is also effective in inducing apoptosis in in vitro functional studies. Please visit the following website to view a protocol for apoptosis induction with EOS9.1:
http://www.ebioscience.com/ebioscience/appls/Apop.htm

Applications Tested

The EOS9.1 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes and has also been tested for its ability to induce apoptosis of Jurkat cells. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kishimoto, T., H. Kikutani, et.al., eds. 1998. Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing Inc. London