Product Information

Contents: Functional Grade Purified anti-mouse CD90 (Thy-1, Thy1)
Catalog Number: 16-0901
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: G7
Isotype: Rat IgG2c, κ

Surface staining of mouse splenocytes with anti-mouse CD90 (G7) Purified followed by anti-rat IgG FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0901	Affinity Purified anti-mouse CD90 (Thy-1, Thy1)	N/A	N/A	FA FC IHC
16-0901	Functional Grade* Purified anti-mouse CD90 (Thy-1, Thy1)	N/A	N/A	FA FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The G7 monoclonal antibody reacts with mouse CD90 also known as Thy-1, a GPI-linked membrane molecule. CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes and mature T cells as well as neurons. CD90 is involved in regulation of adhesion and signal transduction by T cells. G7 is reported to induce TCR-mediated proliferation of T cell and apoptosis of thymocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The G7 antibody has been reported for use in flow cytometric analysis. G7 has also been reported in functional studies.

Applications Tested

The G7 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Gui M, Wiest DL, Li J, Kappes D, Hardy RR, Hayakawa K. Peripheral CD4+ T cell maturation recognized by increased expression of Thy-1/CD90 bearing the 6C10 carbohydrate epitope. J Immunol. 1999 Nov 1;163(9):4796-804. (in vitro stimulation)
Gunter, K. C., T. R. Malek, et al. (1984). "T cell-activating properties of an anti-Thy-1 monoclonal antibody. Possible analogy to OKT3/Leu-4." J Exp Med 159(3): 716-30.

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