Surface staining of mouse splenocytes with anti-mouse CD81 (Eat-2) Biotin followed by SA-PE. Autofluorescence is shown via open histogram. Total viable cells were used for analysis.
Contents: Functional Grade Purified anti-mouse CD81 (TAPA-1) Catalog Number: 16-0811 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, No NaN3 Clone: Eat-2 (EAT2) Host/Isotype: Armenian Hamster IgG Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles. KEEP CONTENTS STERILE. Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Product Options
Cat. No.
Size
Price
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16-0811-81
50 ug
16-0811-82
100 ug
16-0811-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The Eat-2 (EAT2) monoclonal antibody reacts with mouse CD81, a molecule also known as TAPA-1. CD81 is expressed on CD4+CD8+ thymocytes and broadly in the periphery with high level expressed by B cells, NK cells, macrophages and dendritic cells and lower level on resting T cells. Activation of T cells induces upregulation of CD81. CD81 is involved in homotypic adhesion of B and T cells as well as costimulation of T cells. The Eat-2 antibody is reported to cross-react with rat CD81.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The Eat-2 (EAT2) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB).
Applications Tested
The Eat-2 (EAT2) antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Maecker, H. T., S. C. Todd, et al. (2000). "Differential expression of murine CD81 highlighted by new anti-mouse CD81 monoclonal antibodies." Hybridoma 19(1): 15-22.
