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Anti-Mouse CD81 Functional Grade Purified

Also known as: TAPA-1, TAPA1

RUO: For Research Use Only
mouse CD81 antibody, Functional Grade Purified conjugateSurface staining of mouse splenocytes with Anti-Mouse CD81 Biotin followed by Streptavidin PE. Autofluorescence is shown via open histogram. Total viable cells were used for analysis.
Contents: Anti-Mouse CD81 Functional Grade Purified
Catalog Number: 16-0811
Concentration: 1 mg/ml
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN3
Storage Conditions: Store at 2-8°C.
Handling Conditions: Use in sterile envrioment.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: Eat-2 (EAT2)
Host/Isotype: Armenian Hamster IgG
 
Product Options
Cat. No. Size Price Add Qty to Cart
16-0811-81 50 ug
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16-0811-82 100 ug
16-0811-85 500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
 
Research Related Products
Cat. No.Format
11-4111 Anti-Armenian Hamster IgG FITC
11-4317 Streptavidin FITC
12-4317 Streptavidin PE
13-4113 Anti-Armenian Hamster IgG Biotin (Polyclonal)
16-4888 Armenian Hamster IgG Isotype Control Functional Grade Purified (eBio299Arm)
17-4317 Streptavidin APC
 

Available Formats
Cat. No.FormatExcite
(nm)
Emit
(nm)
Reported ApplicationsRegulatory Status
13-0811 Anti-Mouse CD81 Biotin N/A N/A FC RUO
14-0811 Anti-Mouse CD81 Purified N/A N/A FC IP WB RUO
16-0811 Anti-Mouse CD81 Functional Grade* Purified N/A N/A FC IP WB RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
  Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
 
Description
The Eat-2 (EAT2) monoclonal antibody reacts with mouse CD81, a molecule also known as TAPA-1. CD81 is expressed on CD4+CD8+ thymocytes and broadly in the periphery with high level expressed by B cells, NK cells, macrophages and dendritic cells and lower level on resting T cells. Activation of T cells induces upregulation of CD81. CD81 is involved in homotypic adhesion of B and T cells as well as costimulation of T cells. The Eat-2 antibody is reported to cross-react with rat CD81.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The Eat-2 (EAT2) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB).
Applications Tested
The Eat-2 (EAT2) antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References

Maecker, H. T., S. C. Todd, et al. (2000). "Differential expression of murine CD81 highlighted by new anti-mouse CD81 monoclonal antibodies." Hybridoma 19(1): 15-22.

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Unless indicated otherwise, all products are For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for further distribution without written consent. Not all products available in all regions.