Product Information

Contents: Functional Grade Purified anti-mouse CD80 (B7-1)
Catalog Number: 16-0801
Sizes: 50 ug, 100 ug, 500 ug, 1 mg
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: 16-10A1
Isotype: Armenian Hamster IgG

Surface staining of LPS stimulated splenocytes with anti-mouse CD80 (16-10A1) FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0801	Affinity Purified anti-mouse CD80 (B7-1, B71)	N/A	N/A	FA FC IH/F IP
16-0801	Functional Grade* Purified anti-mouse CD80 (B7-1, B71)	N/A	N/A	FA FC
13-0801	Biotin anti-mouse CD80 (B7-1, B71)	N/A	N/A	FC
11-0801	FITC anti-mouse CD80 (B7-1, B71)	488	518	FC
12-0801	PE anti-mouse CD80 (B7-1, B71)	488	575	FC
15-0801	Phycoerythrin-Cy5 (PE-Cy5) anti-mouse CD80 (B7-1, B71)	488	670	FC
17-0801	APC anti-mouse CD80 (B7-1, B71)	633	660	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 16-10A1 antibody has been reported for use in flow cytometric analysis. 16-10A1 has also been reported in blocking of CD80 to its ligands.

Applications Tested

The 16-10A1 antibody has been tested by flow cytometric analysis of activated mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Zhang B, Wang Z, Ding J, Peterson P, Gunning WT, Ding HF. NF-kappaB2 is required for the control of autoimmunity by regulating the development of medullary thymic epithelial cells. J Biol Chem. 2006 Dec 15;281(50):38617-24. (16-10A1, IHC frozen)

Odobasic D, Kitching AR, Semple TJ, Timoshanko JR, Tipping PG, Holdsworth SR. Glomerular expression of CD80 and CD86 is required for leukocyte accumulation and injury in crescentic glomerulonephritis. J Am Soc Nephrol. 2005 Jul;16(7):2012-22. (16-10A1, FA and IHC frozen)

Hancock WW, Sayegh MH, Zheng XG, Peach R, Linsley PS, Turka LA. Costimulatory function and expression of CD40 ligand, CD80, and CD86 in vascularized murine cardiac allograft rejection. Proc Natl Acad Sci U S A. 1996 Nov 26;93(24):13967-72. (16-10A1, IHC frozen)

Razi-Wolf Z, Falo LD Jr, Reiser H. Expression and function of the costimulatory molecule B7 on murine Langerhans cells: evidence for an alternative CTLA-4 ligand. Eur J Immunol. 1994 Apr;24(4):805-11.

Galvin F, Freeman GJ, Razi-Wolf Z, Hall W Jr, Benacerraf B, Nadler L, Reiser H. Murine B7 antigen provides a sufficient costimulatory signal for antigen-specific and MHC-restricted T cell activation. J Immunol. 1992 Dec 15;149(12):3802-8.

Razi-Wolf Z, Freeman GJ, Galvin F, Benacerraf B, Nadler L, Reiser H. Expression and function of the murine B7 antigen, the major costimulatory molecule expressed by peritoneal exudate cells. Proc Natl Acad Sci U S A. 1992 May 1;89(9):4210-4.

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