Product Information

Contents: Functional Grade Purified anti-human CD62L (L-selectin)
Catalog Number: 16-0629
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: DREG-56 (DREG56)
Isotype: Mouse IgG1, κ
HLDA No.: V S056

Surface staining of normal human peripheral blood cells with anti-human CD62L (DREG-56 ) FITC (left), and APC (right). Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0629	Affinity Purified anti-human CD62L (L-selectin)	N/A	N/A	FA FC IH/F WB
16-0629	Functional Grade* Purified anti-human CD62L (L-selectin)	N/A	N/A	FA FC
11-0629	FITC anti-human CD62L (L-selectin)	488	518	FC
12-0629	PE anti-human CD62L (L-selectin)	488	575	FC
15-0629	PE-Cy5 anti-human CD62L (L-selectin)	488	670	FC
17-0629	Allophycocyanin (APC) anti-human CD62L (L-selectin)	633	660	FC
25-0629	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD62L (L-selectin)	488	760	FC
27-0629	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD62L (L-selectin) (APC-Cy7 Equivalent)	633	779	FC
93-0629	eFluor^TM 605^NC anti-human CD62L (L-selectin)	350-500	605	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The DREG-56 monoclonal antibody reacts with human CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCAM-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The DREG-56 (DREG56) antibody has been reported for use in flow cytometric analysis. DREG-56 has also been reported in inhibition of binding to HEV.

Applications Tested

The DREG-56 (DREG56) antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Schlossman, S., L. Bloumsell, et al. eds. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

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