anti-mouse CD61 antibody: Functional Grade - no azide, azide free antibody

Contents: Functional Grade Purified anti-mouse CD61
Catalog Number: 16-0611
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: 2C9.G3
Isotype: Armenian Hamster IgG

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
16-0611-81	50 ug	$90
16-0611-82	100 ug	$160
16-0611-85	500 ug	$305

*International customers: Please contact your distributor for region specific pricing.

Surface staining of mouse bone marrow with anti-mouse CD61 (2C9.G3) FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Cells in the myeloid population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0611	FITC anti-mouse CD61 (Integrin beta 3)	488	518	FC
12-0611	PE anti-mouse CD61 (Integrin beta 3)	488	575	FC
13-0611	Biotin anti-mouse CD61 (Integrin beta 3)	N/A	N/A	FC
14-0611	Affinity Purified anti-mouse CD61 (Integrin beta 3)	N/A	N/A	FA FC IH/F
16-0611	Functional Grade* Purified anti-mouse CD61 (Integrin beta 3)	N/A	N/A	FA FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 2C9.G3 (HMb3-1) monoclonal antibody reacts with mouse CD61, also known as the integrin β₃. CD61 is expressed by activated T cells, granulocytes, and platelet. CD61 associates non-covalently with two integrin α subunits; α_V (CD51) to form Vitronectin Receptor and α_Iib (CD41) to form gpIIb/IIIa. These heterodimeric complexes are responsible for adhesion to extracellular matrix components including fibrinogen, fibronectin, fibronectin, vitronectin, thrombospondin and von Willebrand factor.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 2C9.G3 antibody has been reported for use in flow cytometric analysis. 2C9.G3 has also been reported in blocking of ligand binding and some adhesive processes.

Applications Tested

The 2C9.G3 antibody has been tested by flow cytometric analysis of mouse splenocyte and bone marrow cell suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Yasuda M, Hasunuma Y, Adachi H, Sekine C, Sakanishi T, Hashimoto H, Ra C, Yagita H, Okumura K. 1995. Expression and function of fibronectin binding integrins on rat mast cells. Int Immunol. 7:251-8.

Nohara K, Pan X, et al. 2005. Constitutively active aryl hydrocarbon receptor expressed specifically in T-lineage cells causes thymus involution and suppresses the immunization-induced increase in splenocytes. J Immunol. 174(5):2770-7. (FC, PubMed)

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