Product Information

Contents: Functional Grade Purified anti-human CD54 (ICAM-1)
Catalog Number: 16-0549
Sizes: 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: HA58
Isotype: Mouse IgG1, κ
HLDA No.: VI A095

Surface staining of normal human peripheral blood cells with anti-human CD54 (HA58) PE. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-0549	PE anti-human CD54 (ICAM-1)	488	575	FC
13-0549	Biotin anti-human CD54 (ICAM-1)	N/A	N/A	FC
14-0549	Affinity Purified anti-human CD54 (ICAM-1)	N/A	N/A	FA FC IHC WB
16-0549	Functional Grade* Purified anti-human CD54 (ICAM-1)	N/A	N/A	FA FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

The HA58 monoclonal antibody reacts with human CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligand CD11a is important in the inflammatory response.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HA58 antibody has been reported for use in flow cytometric analysis. HA58 has also been reported in blocking of in vitro mixed lymphocyte reaction.

Applications Tested

The HA58 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.25 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Knapp, W., B. Dorken, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Schlossman, S., L. Bloumsell, et al. eds 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

Kishimoto, T., H. Kikutani, et al., eds. 1998. Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing Inc. London.

Kanwar JR, Berg RW, et al. 2003. Requirements for ICAM-1 immunogene therapy of lymphoma. Cancer Gene Ther. 10(6):468-76. (WB, PubMed)

Bao J, Sato K, et al. 2001. PR-39 and PR-11 peptides inhibit ischemia-reperfusion injury by blocking proteasome-mediated I kappa B alpha degradation. Am J Physiol Heart Circ Physiol. 281(6):H2612-8. (WB, PubMed)

Davis DM, Chiu I, et al. 1999. The human natural killer cell immune synapse. Proc Natl Acad Sci U S A. 96(26):15062-7. (IHC, PubMed)

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