Product Information

Contents: Functional Grade Purified anti-human CD49d (Integrin α₄)
Catalog Number: 16-0499
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: 9F10
Isotype: Mouse IgG1, κ
HLDA No.: V S215

Surface staining of normal human peripheral blood cells with anti-human CD49d (9F10) PE. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0499	Affinity Purified anti-human CD49d (Integrin alpha 4)	N/A	N/A	FA FC IHC
16-0499	Functional Grade* Purified anti-human CD49d (Integrin alpha 4)	N/A	N/A	FA FC
12-0499	PE anti-human CD49d (Integrin alpha 4)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 9F10 monoclonal antibody reacts with human CD49d, the 150 kDa integrin α₄ subunit. The complex of CD49d non-covalently associated with integrin β₁ (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106). This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils. CD49d also associates with integrin β₇ and binds to the Mucosal Addressin Cell-Adhesion Molecule-1 (MadCAM-1).

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 9F10 antibody has been reported for use in flow cytometric analysis. 9F10 has also been reported in in vitro functional assays.

Applications Tested

The 9F10 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

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