Product Information

Contents: Functional Grade Purified anti-human CD49b (Integrin alpha 2)
Catalog Number: 16-0498
Sizes: 100 ug
Formulation: Phosphate buffered saline, pH 7.2, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENT STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: eBioY418 (Y418)
Isotype: Mouse IgG1, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0498	Affinity Purified anti-human CD49b (Integrin alpha 2)	N/A	N/A	FC
16-0498	Functional Grade* Purified anti-human CD49b (Integrin alpha 2)	N/A	N/A	FA FC
11-0498	Fluorescein isothiocyanate (FITC) anti-human CD49b (Integrin alpha 2)	488	518	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioY418 monoclonal antibody reacts with human CD49b, the 150 kDa integrin α2 subunit. The complex of CD49b non-covalently associated with integrin β1 (CD29), also known as VLA-2, is a receptor for collagen and laminin. This complex is expressed by lymphocytes, activated T cells, monocytes and platelets.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioY418 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioY418 antibody has been tested by flow cytometric analysis of normal human peripheral blood. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Related Products

Cat. 16-0491 Functional Grade Purified anti-mouse CD49b (Integrin alpha 2) (clone HMa2)
Cat. 16-4714 Functional Grade Purified Mouse IgG1, K Isotype Control