Staining of Chinese Hamster Ovary (CHO) cell line with 0.25 µg of Mouse IgG1 κ Isotype Control Purified (cat. 14-4714) (open histogram) or 0.25 µg of Anti-Integrin β5 Purified (filled histogram) followed by Anti-Mouse IgG FITC (cat. 11-4011). Total viable cells were used for analysis.
Contents: Anti-Integrin beta 5 Functional Grade Purified Catalog Number: 16-0497 Concentration: 1 mg/ml Formulation: aqueous buffer, no sodium azide Storage Conditions: Store at 2-8°C. Handling Conditions: Use in sterile envrioment. Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay. Clone: KN52 Host/Isotype: Mouse IgG1, κ
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16-0497-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The KN52 antibody reacts with human integrin beta 5, an approximately 100,000 Mr (reduced) and 95,000 Mr (nonreduced) a member of the integrin family. Only a single alpha chain, the alpha v subunit, associates with the integrin beta 5 to form the vitronectin receptor complex. Integrin alpha v/beta 5 complex also binds to the basic domain of Tat (he sequence RKKRRQRRR). The integrin beta 5 is found on carcinoma cell lines, hepatoma and fibroblast cell lines, and is absent from lymphoblastoid cells and platelets.
The KN52 monoclonal antibody has been shown to inhibit migration.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This KN52 antibody has been reported for use in flow cytometric analysis. The KN52 antibody is also reported to have functional activity.
Applications Tested
This KN52 antibody has been tested by flow cytometric analysis of Chinese Hamster Ovary (CHO), A549 and normal human peripheral blood cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Sangaletti S, Di Carlo E, Gariboldi S, Miotti S, Cappetti B, Parenza M, Rumio C, Brekken RA, Chiodoni C, Colombo MP. Macrophage-derived SPARC bridges tumor cell-extracellular matrix interactions toward metastasis. Cancer Res. 2008 Nov 1;68(21):9050-9. (KN52, FA, PubMed)
Lai CF, Feng X, Nishimura R, Teitelbaum SL, Avioli LV, Ross FP, Cheng SL. Transforming growth factor-beta up-regulates the beta 5 integrin subunit expression via Sp1 and Smad signaling. J Biol Chem. 2000 Nov 17;275(46):36400-6.
Sugiyama M, Speight PM, Prime SS, Watt FM. Comparison of integrin expression and terminal differentiation capacity in cell lines derived from oral squamous cell carcinomas. Carcinogenesis. 1993 Oct;14(10):2171-6.
