Contents: Functional Grade Purified anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)
Catalog Number: 16-0451
Formulation: Phosphate buffered saline, pH 7.2, No NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENT STERILE.
Clone: 30-F11
Isotype: Rat IgG2b, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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Staining of C57Bl/6 splenocytes with 0.25 μg of Purified Rat IgG2b Iso Cntrl (cat. 14-4331) (open histogram) or 0.25 μg of Purified anti-mouse CD45 (30-F11) (colored histogram) followed by FITC Anti-Rat IgG (cat. 11-4811). Cells in the lymphocyte gate were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-0451 |
FITC anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) |
488 |
518 |
FC
|
| 12-0451 |
PE anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) |
488 |
575 |
FC
|
| 13-0451 |
Biotin anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) |
N/A |
N/A |
FC
|
| 14-0451 |
Affinity Purified anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) |
N/A |
N/A |
FA
FC
IHC
IP
|
| 15-0451 |
PE-Cy5 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) |
488 |
670 |
FC
|
| 16-0451 |
Functional Grade* Purified anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) |
N/A |
N/A |
FA
FC
IHC
IP
|
| 17-0451 |
APC anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) |
633 |
660 |
FC
|
| 25-0451 |
Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) |
488 |
760 |
FC
|
| 27-0451 |
Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) (APC-Cy7 Equivalent) |
633 |
779 |
FC
|
| 35-0451 |
PE-Cy5.5 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) |
488 |
690 |
FC
|
| 45-0451 |
PerCP-Cy5.5 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) |
488 |
690 |
FC
|
| 56-0451 |
Alexa Fluor® 700 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) (APC-Cy5.5 Equivalent) |
633 |
723 |
FC
|
| 57-0451 |
Pacific Blue® anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) |
402 |
421 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 30-F11 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. 30-F11 has also been reported in complement-dependent cytotoxicity. (Please use Functional Grade purified 30-F11 in functional assays.)
Applications Tested
This 30-F11 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Ledbetter, J. A. and L. A. Herzenberg (1979). "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens."
Immunol Rev 47: 63-90.
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Mouse Cellular Antigens Chart