Contents: Functional Grade Purified anti-mouse/human CD44 (Pgp-1, Ly-24)
Catalog Number: 16-0441
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: IM7
Isotype: Rat IgG2b, κ
|
|
Staining of BALB/c splenocytes with 0.06 μg FG Purified Rat IgG2b Iso Cntrl (cat. 16-4331) (open histogram) or 0.06 μg FG Purified anti-mouse/human CD44 (IM7) (colored histogram) followed by FITC Anti-Rat IgG (cat. 11-4811). Total viable cells were used for analysis.
|
| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-0441 |
Affinity Purified anti-mouse/human CD44 (Pgp-1, Ly-24) |
N/A |
N/A |
FA
FC
IHC
IP
WB
|
| 16-0441 |
Functional Grade* Purified anti-mouse/human CD44 (Pgp-1, Ly-24) |
N/A |
N/A |
FA
FC
|
| 13-0441 |
Biotin anti-mouse/human CD44 (Pgp-1, Ly-24) |
N/A |
N/A |
FC
|
| 11-0441 |
FITC anti-mouse/human CD44 (Pgp-1, Ly-24) |
488 |
518 |
FC
|
| 12-0441 |
PE anti-mouse/human CD44 (Pgp-1, Ly-24) |
488 |
575 |
FC
|
| 15-0441 |
PE-Cy5 anti-mouse/human CD44 (Pgp-1, Ly-24) |
488 |
670 |
FC
|
| 17-0441 |
APC anti-mouse/human CD44 (Pgp-1, Ly-24) |
633 |
660 |
FC
|
| 25-0441 |
Phycoerythrin-Cy7 (PE-Cy7) anti-mouse/human CD44 (Pgp-1, Ly-24) |
488 |
760 |
FC
|
| 35-0441 |
PE-Cy5.5 anti-mouse/human CD44 (Pgp-1, Ly-24) |
488 |
690 |
FC
|
| 56-0441 |
Alexa Fluor® 700 anti-mouse/human CD44 (Pgp-1, Ly-24) (APC-Cy5.5 Equivalent) |
633 |
723 |
FC
|
| 45-0441 |
PerCP-Cy5.5 anti-mouse/human CD44 (Pgp-1, Ly-24) (PE-Cy5.5 replacement) |
488 |
690 |
FC
|
| 93-0441 |
eFluorTM 605NC anti-mouse/human CD44 (Pgp-1, Ly-24) |
350-500 |
605 |
FC
|
| 47-0441 |
Allophycocyanin-eFluorTM 780 anti-mouse/human CD44 (Pgp-1, Ly-24) (APC-Alexa Fluor® 750 replacement) |
633 |
780 |
FC
|
| 48-0441 |
eFluorTM 450 anti-mouse/human CD44 (Pgp-1, Ly-24) (Pacific Blue® replacement) |
405 |
450 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen and peripheral B and T cells can upregulate the expression of CD44. CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The IM7 antibody has been reported for use in flow cytometric analysis. IM7 has also been reported in complement-dependent cytotoxicity.
Applications Tested
The IM7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocyte suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Trowbridge, I. S., J. Lesley, et al. 1982.Biochemical characterization and cellular distribution of a polymorphic, murine cell-surface glycoprotein expressed on lymphoid tissues. Immunogenetics 15(3): 299-312.
Lesley, J. and I. S. Trowbridge 1982. Genetic characterization of a polymorphic murine cell-surface glycoprotein. Immunogenetics 15(3): 313-20.
Maiti A, Maki G, Johnson P. TNF-alpha induction of CD44-mediated leukocyte adhesion by sulfation.
Science. 1998. Oct 30;282(5390):941-3.
Related Products
Cat. 16-4031 Functional Grade Purified Rat IgG2b Isotype ControlCat. 11-4317 Streptavidin-FITC (Fluorescein isothiocyanate)Cat. 12-4317 Streptavidin-PE (Phycoerythrin)Cat. 17-4317 Streptavidin Allophycocyanin (SA-APC)Cat. 11-4811 FITC Anti-Rat IgGCat. 13-4813 Biotin Anti-Rat IgG (clone Polyclonal)
Mouse Cellular Antigens Chart
Human Cellular Antigens Chart