Product Information

Contents: Functional Grade Purified anti-mouse CD43 (Leukosialin)
Catalog Number: 16-0431
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENT STERILE.
Clone: eBioR2/60
Isotype: Rat IgM

Staining of C57Bl/6 splenocytes with .25 μg of Purified Rat IgM Iso Cntrl (cat. 14-4341) (open histogram) or .25 μg of Purified anti-mouse CD43 (eBioR2/60) (colored histogram) followed by PE anti-rat IgM (HIS40) (cat. 12-0990). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0431	Affinity Purified anti-mouse CD43 (Leukosialin)	N/A	N/A	FC IP WB
16-0431	Functional Grade* Purified anti-mouse CD43 (Leukosialin)	N/A	N/A	FA FC IP WB
13-0431	Biotin anti-mouse CD43 (Leukosialin)	N/A	N/A	FC
11-0431	Fluorescein isothiocyanate (FITC) anti-mouse CD43 (Leukosialin)	488	518	FC
12-0431	Phycoerythrin (PE) anti-mouse CD43 (Leukosialin)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioR2/60 monoclonal antibody reacts with mouse CD43, also known as leukosialin and sialophorin. CD43 has glycoforms of approximately 115 and 130 kDa, and is expressed by an early B cell subset in BM, thymocytes and mature T cells, NK cells, myeloid lineage cells and platelets. CD43 is reported to bind to CD54 and play a role in adhesion and B cell survival.

While the S7 and 1B11 monoclonal antibody clones detect only certain isoforms of CD43, data suggests that R2/60 detects the expression of both isoforms. It has been demonstrated that R2/60 stains the majority of CD4+ and CD8+ splenocytes, as well as a significant population of CD19+ splenocytes. However, the clones S7 and 1B11 stain only CD4+ and CD8+, or CD19+ splenocytes, respectively. This suggests that S7 and 1B11 recognize distinct epitopes which are differentially expressed by splenic B and T cells, whereas R2/60 recognizes an epitope shared by both isoforms of CD43.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioR2/60 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB). R2/60 has also been reported to augment the proliferative response induced through T cell receptor mediated activation.

Applications Tested

This eBioR2/60 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Bagriacik EU, Armstrong MD, Okabe M, Klein JR. Differential expression of CD43 isoforms on murine T cells and their relationship to acute intestinal graft versus host disease: studies using enhanced-green fluorescent protein transgenic mice. Int Immunol. 1999 Oct;11(10):1651-62. (R2/60, WB, FC, PubMed)

Sperling AI, Green JM, Mosley RL, Smith PL, DiPaolo RJ, Klein JR, Bluestone JA, Thompson CB. CD43 is a murine T cell costimulatory receptor that functions independently of CD28. J Exp Med. 1995 Jul 1;182(1):139-46. (R2/60, FC, WB, FA, PubMed)

Related Products

Cat. 11-0990  FITC anti-rat IgM (clone HIS40)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 16-4341  Functional Grade Purified Rat IgM Isotype Control