Staining of mouse splenocytes with Anti-Mouse CD40 PE (left)or APC (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
Contents: Anti-Mouse CD40 Functional Grade Purified Catalog Number: 16-0401 Concentration: 1 mg/ml Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, No NaN3 Storage Conditions: Store at 2-8°C. Handling Conditions: Use in sterile envrioment. Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay. Clone: 1C10 Host/Isotype: Rat IgG2a, κ
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16-0401-81
50 ug
16-0401-82
100 ug
16-0401-85
500 ug
16-0401-86
1 mg
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The 1C10 monoclonal antibody reacts with mouse CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development/maturation by inducing Ig isotype switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 1C10 antibody has been reported for use in flow cytometric analysis. It has also been reported in in vitro activation of B cells.
Applications Tested
The 1C10 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Ferlin, W. G., T. von der Weid, et al. (1998). "The induction of a protective response in Leishmania major-infected BALB/c mice with anti-CD40 mAb." Eur J Immunol 28(2): 525-31. Ferlin, W. G., E. Severinson, et al. (1996). "CD40 signaling induces interleukin-4-independent IgE switching in vivo." Eur J Immunol 26(12): 2911-5. Heath, A. W., W. W. Wu, et al. (1994). "Monoclonal antibodies to murine CD40 define two distinct functional epitopes." Eur J Immunol 24(8): 1828-34.
