Staining of normal human peripheral blood cells with APC anti-human CD19 (HIB19) (cat. 17-0199) and 0.5 μg of FG Purified Mouse IgG1, K Iso Cntrl (cat. 12-4714) (left) or 0.5 μg of FG Purified anti-human CD32 (6C4) (right) followed by PE Donkey F(ab')2 Fragment Anti-Mouse IgG (H+L)(Minimal Reactivity to Rat IgG) (cat. 12-4012). Cells in the lymphocyte gate were used for analysis.
Contents: Functional Grade Purified anti-human CD32 Catalog Number: 16-0329 Formulation: aqueous buffer, pH 7.2, No NaN3 Clone: 6C4 (CD32) Host/Isotype: Mouse IgG1, k Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles. KEEP CONTENT STERILE.
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Cat. No.
Size
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16-0329-81
50 ug
16-0329-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
This 6C4 monoclonal antibody reacts with human CD32 (also known as FcγRII). This antibody recognizes two isoforms of the receptor, FcγRIIA and FcγRIIB. These 40-kDa polymorphic transmembrane glycoproteins are expressed on B cells, granulocytes, monocytes, macrophages, and platelets. Moreover, these receptors are detected on natural killer cells. CD32 enables interaction between FcγRII-expressing cells and opsonized antigen or IgG-containing immune complexes. Both receptors exhibit low affinity towards IgG and play a role in inflammation and autoimmune disease. This clone has been reported to inhibit Ig binding in a rosette blocking assay.
Applications Reported
For research use only, not for diagnostic or therapeutic use.
Applications Tested
This 6C4 (CD32) antibody has been tested by flow cytometric analysis on lysed whole blood. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Fossati G, Bucknall RC, and Edwards SW. Fcγ receptors in autoimmune diseases. Eur. J. Clin. Invest. 2001 31(9):821-831.
Vely F, Gruel N, Moncuit J, Cochet O, Rouard H, Dare S, Galon J, Sautes C, Fridman WH, Teillaud JL. A new set of monoclonal antibodies against human Fc gamma RII (CD32) and Fc gamma RIII (CD16): characterization and use in various assays. Hybridoma 1997 Dec; 16(6):519-28. (6C4, FC, FA (blocking), Pubmed)
