Product Information

Contents: Functional Grade Purified anti-human CD31 (PECAM-1)
Catalog Number: 16-0319
Sizes: 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: WM-59 (WM59)
Isotype: Mouse IgG1, κ
HLDA No.: V P025

Surface staining of normal human peripheral blood cells with anti-human CD31 (WM-59) FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Cells in the monocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0319	FITC anti-human CD31 (PECAM-1)	488	518	FC
12-0319	PE anti-human CD31 (PECAM-1)	488	575	FC
13-0319	Biotin anti-human CD31 (PECAM-1)	N/A	N/A	FC
14-0319	Affinity Purified anti-human CD31 (PECAM-1)	N/A	N/A	FC IH/F
16-0319	Functional Grade* Purified anti-human CD31 (PECAM-1)	N/A	N/A	FC
17-0319	APC anti-human CD31 (PECAM-1)	633	660	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

The WM59 monoclonal antibody reacts with human CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. This 130-140 kDa surface protein is expressed by endothelial cells and at low levels on leukocytes and platelets. It has been reported that CD38 binds to CD31. Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interaction, and signal transduction.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The WM-59 (WM59) antibody has been reported for use in flow cytometric analysis.

Applications Tested

The WM-59 (WM59) antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.25 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Schlossman, S., L. Bloumsell, et al. eds. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

Kishimoto, T., A.E.G., von dem Borne, et al. eds. (1998) Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing, Inc. London.

Porat Y, Porozov S, Belkin D, Shimoni D, Fisher Y, Belleli A, Czeiger D, Silverman WF, Belkin M, Battler A, Fulga V, Savion N Isolation of an adult blood-derived progenitor cell population capable of differentiation into angiogenic, myocardial and neural lineages. British Journal of Haematology 2006 (in process) (Immunocytochemistry)

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