Contents: Functional Grade Purified anti-mouse CD29 (Integrin beta 1)
Catalog Number: 16-0291
Formulation: Phosphate buffered saline, pH 7.2, No NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENT STERILE.
Clone: eBioHMb1-1 (HMb1-1)
Isotype: Armenian Hamster IgG
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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Staining of C57Bl/6 thymocytes with 0.5 μg of Purified Armenian Hamster IgG Iso Cntrl (cat. 14-4888) (open histogram) or 0.5 μg of Purified anti-mouse CD29 (HMb1-1) (colored histogram) followed by Biotin Anti-Armenian Hamster IgG (cat. 13-4113)and SAv-PE (cat. 12-4317). Total viable cells were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 12-0291 |
Phycoerythrin (PE) anti-mouse CD29 (Integrin b1 chain, a4b1, VLA-4) |
488 |
575 |
FC
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| 14-0291 |
Affinity Purified anti-mouse CD29 (Integrin b1 chain, a4b1, VLA-4) |
N/A |
N/A |
FA
FC
IP
|
| 16-0291 |
Functional Grade* Purified anti-mouse CD29 (Integrin b1 chain, a4b1, VLA-4) |
N/A |
N/A |
FA
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The eBioHMb1-1 monoclonal antibody reacts with mouse CD29 (integrin β
1), a 110-120 kDa member of the β integrin family expressed by leukocytes, endothelial, smooth muscle and epithelial cells. CD29 binds non-covalently with the α integrins CD49a-f, to form the VLA-1 through VLA-6 complexes, respectively, and also with CD51. These αβ integrin heterodimers are capable of mediating a variety of cellular responses including adhesion, trafficking, proliferaton and differentiation. All of the integrins which include CD29 bind to extracellular matrix proteins including collagen, laminin, fibronectin and vitronectin, whereas some CD29-containing integrins also interact with cellular receptors including VCAM-1 and MadCAM-1.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBioHMb1-1 (HMb1-1) antibody has been reported for use in flow cytometric analysis. The HMb1-1 monoclonal antibody has been reported to block VLA-dependent cellular functions, including the adhesion of mouse tumor cell lines to extracellular matrix proteins, and splenic T-cell proliferation induced by anti-CD3 monoclonal antibody.
Applications Tested
This eBioHMb1-1 (HMb1-1) antibody has been tested by flow cytometric analysis of mouse spleen, thymus and bone marrow cell suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Noto K, Kato K, Okumura K, Yagita H.
Identification and functional characterization of mouse CD29 with a mAb.
Int Immunol. 1995 May;7(5):835-42. (
HMβ1-1, mAb development, IP, FA,
PubMed)
Ridger VC, Wagner BE, Wallace WA, Hellewell PG.
Differential effects of CD18, CD29, and CD49 integrin subunit inhibition on neutrophil migration in pulmonary inflammation.
J Immunol. 2001 Mar 1;166(5):3484-90. (
HMβ1-1, FA,
PubMed)
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