Product Information

Contents: Functional Grade Purified anti-mouse CD28
Catalog Number: 16-0281
Sizes: 50 ug, 100 ug, 500 ug, 1 mg
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: 37.51
Isotype: Golden Syrian Hamster IgG

Staining of BALB/c thymocytes with 0.25 μg of Purified Golden Syrian Hamster IgG Iso Cntrl (cat. 14-4914) (open histogram) or 0.25 μg of Purified anti-mouse CD28 (37.51) (colored histogram) followed by FITC Anti-Syrian Hamster IgG (cat. 11-4211). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0281	Affinity Purified anti-mouse CD28	N/A	N/A	FA FC IHC IP
16-0281	Functional Grade* Purified anti-mouse CD28	N/A	N/A	FA FC
13-0281	Biotin anti-mouse CD28	N/A	N/A	FC
12-0281	PE anti-mouse CD28	488	575	FC
15-0281	PE-Cy5 anti-mouse CD28	488	670	FC
17-0281	APC anti-mouse CD28	633	660	FC
25-0281	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD28	488	760	FC
45-0281	PerCP-Cy5.5 anti-mouse CD28 (PE-Cy5.5 replacement)	488	690	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 37.51 monoclonal antibody reacts with the mouse CD28 molecule, a 45 kDa homodimer expressed by thymocytes, mature T cells and NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 37.51 antibody has been reported for use in flow cytometric analysis. 37.51 has also been reported in costimulation of T cells in vitro and in vivo.

Applications Tested

The 37.51 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Nandi, D., J. A. Gross, et al. (1994). "CD28-mediated costimulation is necessary for optimal proliferation of murine NK cells." J Immunol 152(7): 3361-9.
Gross, J. A., E. Callas, et al. (1992). "Identification and distribution of the costimulatory receptor CD28 in the mouse." J Immunol 149(2): 380-8.
Harding, F. A., J. G. McArthur, et al. (1992). "CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones." Nature 356(6370): 607-9.
Gross, J. A., T. St. John, et al. (1990). "The murine homologue of the T lymphocyte antigen CD28. Molecular cloning and cell surface expression." J Immunol 144(8): 3201-10.

Related Products

Cat. 11-4211  FITC Anti-Syrian Hamster IgG (clone Polyclonal)
Cat. 13-4213  Biotin Anti-Syrian Hamster IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 16-4914  Functional Grade Purified Golden Syrian Hamster IgG Isotype Control (clone n/a)