anti-mouse/rat/human CD27 antibody: Functional Grade

Contents: Functional Grade Purified anti-mouse/human/rat CD27
Catalog Number: 16-0271
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: LG.7F9
Isotype: Armenian Hamster IgG
HLDA No.: N/A

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
16-0271-81	50 ug	$90
16-0271-82	100 ug	$160
16-0271-85	500 ug	$300

*International customers: Please contact your distributor for region specific pricing.

Staining of BALB/c splenocytes with 0.25 µg Purified hamster IgG isotype control (cat.14-4888) (open histogram) or 0.25 µg FG Purified anti-human/mouse/rat CD27 (LG.7F9) (colored histogram) followed by FITC anti-Armenian hamster IgG (cat.11-4111). Total viable cells were analyzed.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0271	Affinity Purified anti-mouse, human, rat CD27	N/A	N/A	FA FC IP
16-0271	Functional Grade* Purified anti-mouse, human, rat CD27	N/A	N/A	FA FC
13-0271	Biotin anti-mouse, human, rat CD27	N/A	N/A	FC
11-0271	FITC anti-mouse, human, rat CD27	488	518	FC
12-0271	PE anti-mouse, human, rat CD27	488	575	FC
17-0271	APC anti-mouse, human, rat CD27	633	660	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46^-CD8^- precursors. CD27 binds to CD70 and, through this interaction, plays an important role in T cell-B cell interaction. It has been reported that triggering CD27 plays an important role in the maturation of CD4⁺ and CD8⁺ effector cells. LG.7F9 cross-reacts with human and rat CD27.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The LG.7F9 antibody has been reported for use in flow cytometric analysis. LG.7F9 has also been reported in blocking of ligand binding.

Applications Tested

The LG.7F9 antibody has been tested by flow cytometric analysis of mouse splenocyte cell suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Gravestein LA, Nieland JD, Kruisbeek AM, Borst J. 1995. Novel mAbs reveal potent co-stimulatory activity of murine CD27. International Immunology 7(4): 551-557
Tesselaar K, Gravenstine LA, et al. 1997. Characterization of Murine CD70, the Ligand of the TNF Receptor Family Member CD27. The Journal of Immunology 159: 4959-4965
Tesselaar K, Xiao Y, et al. 2002. Expression of the Murine CD27 Ligand CD70 in Vitro and In Vivo. The Journal of Immunology 170: 33-40

Related Products

Cat. 11-4111    FITC Anti-Armenian Hamster IgG
Cat. 13-4113    Biotin Anti-Armenian Hamster IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 16-4444    Functional Grade Purified Armenian Hamster IgG Isotype Control (clone n/a)
Cat. 16-4888    Functional Grade Purified Armenian Hamster IgG Isotype Control (clone eBio299Arm)