Contents: Functional Grade Purified anti-mouse CD25 (IL-2 Receptor α, p55)
Catalog Number: 16-0251
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles
KEEP CONTENTS STERILE
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: PC61.5
Isotype: Rat IgG1, λ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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Staining of 3-day ConA activated C57Bl/6 splenocytes with 0.25 μg of Purified Rat IgG1 Iso Cntrl (cat. 14-4301) (open histogram) or 0.25 μg of Purified anti-mouse CD25 (PC61.5) (colored histogram) followed by FITC Anti-Rat IgG (cat. 11-4811). Total viable cells were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-0251 |
**May be discontinued; see superior product Alexa Fluor® 488** FITC anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55, IL2Ra) |
488 |
518 |
FC
|
| 12-0251 |
PE anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55, IL2Ra) |
488 |
575 |
FC
|
| 13-0251 |
Biotin anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55, IL2Ra) |
N/A |
N/A |
FC
|
| 14-0251 |
Affinity Purified anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55, IL2Ra) |
N/A |
N/A |
FC
IHC
IP
|
| 15-0251 |
PE-Cy5 anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55, IL2Ra) |
488 |
670 |
FC
|
| 16-0251 |
Functional Grade* Purified anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55, IL2Ra) |
N/A |
N/A |
FA
FC
|
| 17-0251 |
APC anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55, IL2Ra) |
633 |
660 |
FC
|
| 25-0251 |
Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55, IL2Ra) |
488 |
760 |
FC
|
| 27-0251 |
Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55) (APC-Cy7 Equivalent) |
633 |
779 |
FC
|
| 36-0251 |
Functional Grade* Biotin anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55, IL2Ra) |
N/A |
N/A |
FC
|
| 45-0251 |
PerCP-Cy5.5 anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55) |
488 |
690 |
FC
|
| 53-0251 |
Alexa Fluor® 488 anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55, IL2Ra) |
488 |
519 |
FC
|
| 56-0251 |
Alexa Fluor® 700 anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55, IL2Ra) (APC-Cy5.5 Equivalent) |
633 |
723 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor α chain (IL-2Rα). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common γ chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of IL-2 receptor is inhibited by the PC61.5 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The PC61.5 antibody has been reported for use in flow cytometric analysis, and
in vitro blocking of mouse CD25 (IL-2Rα, p55).
Applications Tested
The PC61.5 antibody has been tested by flow cytometric analysis of mouse spleen cell suspensions and ConA-activated splenocyte culture. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Lowenthal, J. W., P. Corthesy, et al. (1985)."High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61."
J Immunol 135(6): 3988-94.
Lowenthal, J. W., C. Tougne, et al. (1985). "Antigenic stimulation regulates the expression of IL 2 receptors in a cytolytic T lymphocyte clone."
J Immunol 134(2): 931-9.
Lowenthal, J. W., R. H. Zubler, et al. (1985)."Similarities between interleukin-2 receptor number and affinity on activated B and T lymphocytes."
Nature 315(6021): 669-72.
Hayashi, T, Hasegawa, K, Adachi, C. (2005) Elimination of CD4+CD25+ T cell accelerates the development of glomerulonephritis during the preactive phase in autoimmune-prone female NZB × NZW F1 mice. Int J Exp Path 86: 289-296 (
PC61.5, in vivo depletion,
PubMed)
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Mouse Cellular Antigens Chart