anti-mouse CD24 (HAS, Heat Stable Antigen) antibody: Functional Grade

Contents: Functional Grade Purified anti-mouse CD24 (Heat Stable Antigen)
Catalog Number: 16-0242
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: M1/69
Isotype: Rat IgG2b, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
16-0242-81	50 ug	$90
16-0242-82	100 ug	$160
16-0242-85	500 ug	$280

*International customers: Please contact your distributor for region specific pricing.

Surface staining of mouse splenocytes with anti-mouse CD24 (M1/69) FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0242	FITC anti-mouse CD24 (Heat Stable Antigen)	488	518	FC
12-0242	PE anti-mouse CD24 (Heat Stable Antigen)	488	575	FC
13-0242	Biotin anti-mouse CD24 (Heat Stable Antigen)	N/A	N/A	FC
14-0242	Affinity Purified anti-mouse CD24 (Heat Stable Antigen)	N/A	N/A	FA FC IHC WB
15-0242	Phycoerythrin-Cy5 (PE-Cy5) anti-mouse CD24 (Heat Stable Antigen)	488	670	FC
16-0242	Functional Grade* Purified anti-mouse CD24 (Heat Stable Antigen)	N/A	N/A	FA FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The M1/69 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA). This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. CD24 is a variably glycosylated molecule resulting in heterogeneity of molecular mass of this antigen on cells of different lineages and antibodies to CD24 exhibit subtle differences in staining level on lymphocyte populations. The expression of CD24 has been used to resolve stages of B lymphopoiesis in mouse bone marrow. It has been reported that P-selectin (CD62P) binds to CD24.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The M1/69 antibody has been reported for use in flow cytometric analysis. M1/69 has also been reported in in vitro functional assays.

Applications Tested

The M1/69 antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Springer T, Galfre G, Secher DS, Milstein C. 1978. Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur J Immunol. 8(8):539-51.
Alterman, L. A., I. N. Crispe, et al. 1990. Characterization of the murine heat-stable antigen: an hematolymphoid differentiation antigen defined by the J11d, M1/69 and B2A2 antibodies. Eur J Immunol 20(7): 1597-602.
Wilson, A., L. M. Day, et al. 1988. Subpopulations of mature murine thymocytes: properties of CD4-CD8+ and CD4+CD8- thymocytes lacking the heat-stable antigen. Cell Immunol 117(2): 312-26.
Hunte BE, Capone M, Zlotnik A, Rennick D, Moore TA. 1998. Acquisition of CD24 expression by Lin-CD43+B220(low)ckit(hi) cells coincides with commitment to the B cell lineage. Eur J Immunol. 28(11):3850-6.

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