Product Information

Contents: Functional Grade Purified anti-human CD19
Catalog Number: 16-0199
Sizes: 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: HIB19
Isotype: Mouse IgG1, κ
HLDA No.: V CD19.11

Surface staining of normal human peripheral blood cells with anti-human CD19 (HIB19) FITC (left), and PE (right). Autofluorescence is indicated by open histogram. Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0199	Affinity Purified anti-human CD19	N/A	N/A	FA FC IH/F
16-0199	Functional Grade* Purified anti-human CD19	N/A	N/A	FA FC
13-0199	Biotin anti-human CD19	N/A	N/A	FC
11-0199	FITC anti-human CD19	488	518	FC
12-0199	PE anti-human CD19	488	575	FC
15-0199	PE-Cy5 anti-human CD19	488	670	FC
17-0199	APC anti-human CD19	633	660	FC
25-0199	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD19	488	760	FC
51-0199	Alexa Fluor® 647 anti-human CD19	633	668	FC
53-0199	Alexa Fluor® 488 anti-human CD19	488	519	FC
56-0199	Alexa Fluor® 700 anti-human CD19 (APC-Cy5.5 Equivalent)	633	723	FC
57-0199	Pacific Blue® anti-human CD19	402	421	FC
27-0199	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD19 (APC-Cy7 Equivalent)	633	779	FC
45-0199	PerCP-Cy5.5 anti-human CD19	488	690	FC
93-0199	eFluor^TM 605^NC anti-human CD19	350-500	605	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, Leu13, MHC class II, and CD19 form a multimolecular complex that associates with BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HIB19 antibody has been reported for use in flow cytometric analysis. It has also been reported in in vitro functional studies.

Applications Tested

The HIB19 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

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