Product Information

Contents: Functional Grade Purified anti-mouse CD19
Catalog Number: 16-0193
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffered saline, pH 7.2, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENT STERILE.
Clone: eBio1D3 (1D3)
Isotype: Rat IgG2a, κ

Staining of C57Bl/6 splenocytes with 0.25 μg of FG Purified Rat IgG2a Iso Cntrl (cat. 16-4321) (open histogram) or 0.25 μg of FG Purified anti-mouse CD19 (1D3) (colored histogram) followed by Biotin Anti-Rat IgG (cat. 13-4813)and SAv-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0193	Fluorescein isothiocyanate (FITC) anti-mouse CD19	488	518	FC
12-0193	Phycoerythrin (PE) anti-mouse CD19	488	575	FC
13-0193	Biotin anti-mouse CD19	N/A	N/A	FC
14-0193	Affinity Purified anti-mouse CD19	N/A	N/A	FA FC IH/F IP
15-0193	Phycoerythrin-Cy5 (PE-Cy5) anti-mouse CD19	488	670	FC
16-0193	Functional Grade* Purified anti-mouse CD19	N/A	N/A	FA FC IH/F
25-0193	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD19	488	760	FC
35-0193	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse CD19	488	690	FC
45-0193	PerCP-Cy5.5 anti-mouse CD19	488	690	FC
51-0193	Alexa Fluor® 647 anti-mouse CD19	633	668	FC
56-0193	Alexa Fluor® 700 anti-mouse CD19 (APC-Cy5.5 Equivalent)	633	723	FC
57-0193	Pacific Blue® anti-mouse CD19	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections. The eBio1D3 monoclonal antibody has also been reported to induce down-regulation of CD19, affecting the proportions of B-1a and B-2 B cells in mice.

Applications Tested

This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Cherukuri A, Cheng PC, Pierce SK. The role of the CD19/CD21 complex in B cell processing and presentation of complement-tagged antigens. J Immunol. 2001 Jul 1;167(1):163-72.

Inabe K, Kurosaki T. Tyrosine phosphorylation of B-cell adaptor for phosphoinositide 3-kinase is required for Akt activation in response to CD19 engagement. Blood. 2002 Jan 15;99(2):584-9.

Krop I, de Fougerolles AR, Hardy RR, Allison M, Schlissel MS, Fearon DT. Self-renewal of B-1 lymphocytes is dependent on CD19. Eur J Immunol. 1996 Jan;26(1):238-42. (1D3, FA, PubMed)

Shoham T, Rajapaksa R, Boucheix C, Rubinstein E, Poe JC, Tedder TF, Levy S. The tetraspanin CD81 regulates the expression of CD19 during B cell development in a postendoplasmic reticulum compartment. J Immunol. 2003 Oct 15;171(8):4062-72.

Krop I, Shaffer AL, Fearon DT, Schlissel MS. The signaling activity of murine CD19 is regulated during cell development. J Immunol. 1996 Jul 1;157(1):48-56. (1D3, FC, FA, PubMed)

Related Products

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Cat. 13-0191    Biotin anti-mouse CD19 (clone MB19-1)
Cat. 14-0191    Affinity Purified anti-mouse CD19 (clone MB19-1)
Cat. 16-0191    Functional Grade Purified anti-mouse CD19 (clone MB19-1)
Cat. 17-0191    APC anti-mouse CD19 (clone MB19-1)
Cat. 36-0191    Functional Grade Biotin anti-mouse CD19 (clone MB19-1)
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