Product Information

Contents: Functional Grade Purified anti-mouse CD19
Catalog Number: 16-0192
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENT STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: 6D5
Isotype: Rat IgG2a, κ

Staining of C57Bl/6 splenocytes with 0.06 μg of Purified Rat IgG2a Iso Cntrl (cat. 14-4321) (open histogram) or 0.06 μg of Purified anti-mouse CD19 (6D5) (colored histogram) followed by FITC Anti-Rat IgG (cat. 11-4811). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications

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Description

The 6D5 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together, CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. 6D5 can be used as a phenotypic marker for mouse mature B cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 6D5 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. The 6D5 monoclonal antibody has also been reported for use in functional studies.

Applications Tested

This 6D5 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Krop, I., A. L. Shaffer, et al. (1996). "The signaling activity of murine CD19 is regulated during cell development." J Immunol 157(1): 48-56.
Krop, I., A. R. de Fougerolles, et al. (1996). "Self-renewal of B-1 lymphocytes is dependent on CD19." Eur J Immunol 26(1): 238-42.

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