Contents: Functional Grade Purified anti-human CD11b (Integrin αM, Mac-1) - activation epitope
Catalog Number: 16-0113
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: CBRM1/5
Isotype: Mouse IgG1, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-0113 |
FITC anti-human CD11b (Integrin alpha M, Mac-1) - activation epitope |
488 |
518 |
FC
|
| 12-0113 |
PE anti-human CD11b (Integrin alpha M, Mac-1) - activation epitope |
488 |
575 |
FC
|
| 14-0113 |
Affinity Purified anti-human CD11b (Integrin alpha M, Mac-1) - activation epitope |
N/A |
N/A |
FA
FC
IP
|
| 16-0113 |
Functional Grade* Purified anti-human CD11b (Integrin alpha M, Mac-1) - activation epitope |
N/A |
N/A |
FA
FC
|
| 51-0113 |
Alexa Fluor® 647 anti-human CD11b (Integrin alpha M, Mac-1) - activation epitope |
633 |
668 |
FC
|
| 56-0113 |
Alexa Fluor® 700 anti-human CD11b (Integrin alpha M, Mac-1) - activation epitope (APC-Cy5.5 Equivalent) |
633 |
723 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The CBRM1/5 monoclonal antibody reacts with an activation-specific epitope of human Mac-1. CBRM1/5 binds a subset of Mac-1 molecules on neutrophils and monocytes after stimulation with chemoattractants or phorbol esters but does not recognize Mac-1 on resting myeloid cells. Through interactions with its ligands, Mac-1 participates in adhesive cell interactions. The epitope recognized by this mAb localizes to the I domain on the α chain of Mac-1 very close to the ligand binding site in a region that is widely exposed. CBRM1/5 blocks Mac-1 dependent adhesion to fibrinogen and ICAM-1 and inhibits chemoattractant-stimulated adhesion of eosinophils to the Intercellular Adhesion Molecule-1 (ICAM-1).
Applications Reported
For research use only, not for diagnostic or therapeutic use. The CBRM1/5 antibody has been reported for use in flow cytometric analysis. CBRM1/5 has also been reported in blocking of CD11b function.
Applications Tested
The CBRM1/5 antibody has been tested by flow cytometric analysis of resting and activated human peripheral leukocytes. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Oxvig, C., C. Lu, et al. 1999. Conformational changes in tertiary structure near the ligand binding site of an integrin I domain. Proc Natl Acad Sci U S A 96(5): 2215-20.
Weber, C., J. Kitayama, et al. 1996. Differential regulation of beta 1 and beta 2 integrin avidity by chemoattractants in eosinophils. Proc Natl Acad Sci U S A 93(20): 10939-44.
Diamond, M. S. and T. A. Springer 1993. A subpopulation of Mac-1 (CD11b/CD18) molecules mediates neutrophil adhesion to ICAM-1 and fibrinogen. J Cell Biol 120(2): 545-56.
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Human Cellular Antigens Chart