Product Information

Contents: Functional Grade Purified anti-mouse CD11a (Integrin α_L, LFA-1α)
Catalog Number: 16-0111
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: M17/4
Isotype: Rat IgG2a, κ

Staining of C57Bl/6 splenocytes with 0.25 μg of FG Purified Rat IgG2a Iso Cntrl (cat. 16-4321) (open histogram) or 0.25 μg of FG Purified anti-mouse CD11a (M17/4) (colored histogram) followed by FITC Anti-Rat IgG (cat. 11-4811). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0111	Affinity Purified anti-mouse CD11a (Integrin alpha L, LFA-1 alpha)	N/A	N/A	FA FC IHC IP
16-0111	Functional Grade* Purified anti-mouse CD11a (Integrin alpha L, LFA-1 alpha)	N/A	N/A	FC
13-0111	Biotin anti-mouse CD11a (Integrin alpha L, LFA-1 alpha)	N/A	N/A	FC
11-0111	FITC anti-mouse CD11a (Integrin alpha L, LFA-1 alpha)	488	518	FC
12-0111	PE anti-mouse CD11a (Integrin alpha L, LFA-1 alpha)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The M17/4 monoclonal antibody reacts with mouse CD11a, the 180 kDa integrin α_L, also known as the lymphocyte function associated antigen-1 (LFA-1) α chain. LFA-1, formed by non-covalent association of CD11a with CD18 (integrin β₂), serves as an important adhesion molecule involved in lymphocyte and granulocyte function. CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3) are ligands for LFA-1. CD11a is expressed by all leukocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The M17/4 antibody has been reported for use in flow cytometric analysis. M17/4 has also been reported in blocking of CD11a function.

Applications Tested

The M17/4 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Sanchez-Madrid, F., P. Simon, et al. 1983. Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1. J Exp Med 158(2): 586-602.
Sanchez-Madrid, F., D. Davignon, et al. 1982. Antigens involved in mouse cytolytic T-lymphocyte (CTL)-mediated killing: functional screening and topographic relationship. Cell Immunol 73(1): 1-11.

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