Contents: Functional Grade Purified anti-mouse CD8a (Ly-2)
Catalog Number: 16-0081
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: 53-6.7
Isotype: Rat IgG2a, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
|
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Surface staining of mouse splenocytes with anti-mouse CD8a (53-6.7) FITC (left), and APC (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-0081 |
FITC anti-mouse CD8 (alpha subunit, CD8a, Ly-2) |
488 |
518 |
FC
|
| 12-0081 |
PE anti-mouse CD8 (alpha subunit, CD8a, Ly-2) |
488 |
575 |
FC
|
| 13-0081 |
Biotin anti-mouse CD8 (alpha subunit, CD8a, Ly-2) |
N/A |
N/A |
FC
|
| 14-0081 |
Affinity Purified anti-mouse CD8 (alpha subunit, CD8a, Ly-2) |
N/A |
N/A |
FC
IH/F
IP
|
| 15-0081 |
PE-Cy5 anti-mouse CD8 (alpha subunit, CD8a, Ly-2) |
488 |
670 |
FC
|
| 16-0081 |
Functional Grade* Purified anti-mouse CD8 (alpha subunit, CD8a, Ly-2) |
N/A |
N/A |
FC
|
| 17-0081 |
APC anti-mouse CD8 (alpha subunit, CD8a, Ly-2) |
633 |
660 |
FC
|
| 19-0081 |
Cy5 anti-mouse CD8 (alpha subunit, CD8a, Ly-2) |
633 |
670 |
FC
|
| 25-0081 |
PE-Cy7 anti-mouse CD8 (alpha subunit, CD8a, Ly-2) |
488 |
760 |
FC
|
| 27-0081 |
Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD8 (alpha subunit, CD8a, Ly-2) (APC-Cy7 Equivalent) |
633 |
779 |
FC
|
| 36-0081 |
Functional Grade* Biotin anti-mouse CD8 (alpha subunit, CD8a, Ly-2) |
N/A |
N/A |
FC
IH/F
|
| 45-0081 |
PerCP-Cy5.5 anti-mouse CD8 (alpha subunit, CD8a, Ly-2) |
488 |
690 |
FC
|
| 51-0081 |
Alexa Fluor® 647 anti-mouse CD8 (alpha subunit, CD8a, Ly-2) |
633 |
668 |
FC
|
| 53-0081 |
Alexa Fluor® 488 anti-mouse CD8 (alpha subunit, CD8a, Ly-2) |
488 |
519 |
FC
|
| 56-0081 |
Alexa Fluor® 700 anti-mouse CD8 (alpha subunit, CD8a, Ly-2) (APC-Cy5.5 Equivalent) |
633 |
723 |
FC
|
| 57-0081 |
Pacific Blue® anti-mouse CD8 (alpha subunit, CD8a, Ly-2) |
402 |
421 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 β chain (CD8 αβ) or as a homodimer (CD8 αα). A majority of thymocytes and a subpopulation of mature αβ TCR T cells express CD8 αβ while γδ TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 αα. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 53-6.7 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The 53-6.7 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Ledbetter, J. A. and L. A. Herzenberg (1979). "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens."
Immunol Rev 47: 63-90.
Ledbetter, J. A., R. V. Rouse, et al. (1980). "T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views."
J Exp Med 152(2): 280-95.
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Mouse Cellular Antigens Chart