Product Information

Contents: Functional Grade Purified anti-human CD4 (L3T4)
Catalog Number: 16-0049
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: RPA-T4
Isotype: Mouse IgG1, κ
HLDA No.: IV T114

Profile of functional grade purified mAb RPA-T4 (CD4) staining on human peripheral blood lymphocytes and detection with FITC anti-mouse IgG (cat.no. 11-4011)

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0049	Affinity Purified anti-human CD4	N/A	N/A	FA FC IHC
16-0049	Functional Grade* Purified anti-human CD4	N/A	N/A	FA FC
13-0049	Biotin anti-human CD4	N/A	N/A	FC
11-0049	FITC anti-human CD4	488	518	FC
12-0049	PE anti-human CD4	488	575	FC
15-0049	PE-Cy5 anti-human CD4	488	670	FC
17-0049	APC anti-human CD4	633	660	FC
25-0049	PE-Cy7 anti-human CD4	488	760	FC
45-0049	PerCP-Cy5.5 anti-human CD4 (PE-Cy5.5 replacement)	488	690	FC
95-0049	eFluor^TM 650^NC anti-human CD4	350-500	650	FC
47-0049	Allophycocyanin-eFluor^TM 780 anti-human CD4 (APC-Alexa Fluor® 750 replacement)	633	780	FC
48-0049	eFluor^TM 450 anti-human CD4 (Pacific Blue® replacement)	405	450	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The RPA-T4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). RPA-T4 blocks HIV binding and mixed lymphocyte reaction. The RPA-T4 antibody recognizes a different epitope than the OKT4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The RPA-T4 antibody has been reported for use in flow cytometric analysis. RPA-T4 has also been reported for use in blocking of CD4 activity in in vitro functional assays.

Applications Tested

The RPA-T4 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 11-4011  FITC Anti-Mouse IgG
Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 16-4714  Functional Grade Purified Mouse IgG1, K Isotype Control