Surface staining of normal human peripheral blood cells with anti-human CD3 (UCHT1) PE-Cy5 (left), and APC (right). Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.
Contents: Functional Grade Purified anti-human CD3 Catalog Number: 16-0038 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, No NaN3 Clone: UCHT1 Host/Isotype: Mouse IgG1, κ HLDA Workshop: III 471 Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles. KEEP CONTENTS STERILE. Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
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Size
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16-0038-81
50 ug
16-0038-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits γ and δ, the ε chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The UCHT1 antibody has been reported for use in flow cytometric analysis. UCHT1 has also been reported in activation of T cells.
Applications Tested
The UCHT1 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York. McMichael, A.J., P.C.L. Beverly, et al. eds. (1987). Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.
Baeten D, De Keyser F, et al. 2004. Tumour necrosis factor alpha independent disease mechanisms in rheumatoid arthritis: a histopathological study on the effect of infliximab on rheumatoid nodules. Ann Rheum Dis. 63(5):489-93. (IHC frozen, PubMed)
Senechal B, Elain G, et al. 2007. Expansion of regulatory T cells in patients with Langerhans cell histiocytosis. PLoS Med. 14;4(8):e253. (IHC frozen, PubMed)
Pollard K, Lunny D, et al. 1987. Fixation, processing, and immunochemical reagent effects on preservation of T-lymphocyte surface membrane antigens in paraffin-embedded tissue. J Histochem Cytochem. 35(11):1329-38. (IHC paraffin and frozen, PubMed)
