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Human Cellular Antigens Chart

Contents: Functional Grade Purified anti-human CD3 Catalog Number: 16-0038 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, No NaN3 Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles. KEEP CONTENTS STERILE. Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay. Clone: UCHT1 Isotype: Mouse IgG1, κ HLDA No.: III 471 Prices for This Product* Cat. No. Size Price Qty Action 16-0038-81 50 ug $110 16-0038-85 500 ug $320 *International customers: Please contact your distributor for region specific pricing.

Surface staining of normal human peripheral blood cells with anti-human CD3 (UCHT1) PE-Cy5 (left), and APC (right). Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0038	FITC anti-human CD3	488	518	FC
12-0038	PE anti-human CD3	488	575	FC
13-0038	Biotin anti-human CD3	N/A	N/A	FC
14-0038	Affinity Purified anti-human CD3	N/A	N/A	FA  FC  IH/F
15-0038	PE-Cy5 anti-human CD3	488	670	FC
16-0038	Functional Grade* Purified anti-human CD3	N/A	N/A	FA  FC
17-0038	APC anti-human CD3	633	660	FC
25-0038	PE-Cy7 anti-human CD3	488	760	FC
27-0038	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD3 (APC-Cy7 Equivalent)	633	779	FC
56-0038	Alexa Fluor® 700 anti-human CD3 (APC-Cy5.5 Equivalent)	633	723	FC
57-0038	Pacific Blue® anti-human CD3	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.   Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits γ and δ, the ε chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. The UCHT1 antibody has been reported for use in flow cytometric analysis. UCHT1 has also been reported in activation of T cells.

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Applications Tested

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The UCHT1 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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References

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Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
McMichael, A.J., P.C.L. Beverly, et al. eds. (1987). Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.

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