Contents: Functional Grade Purified anti-mouse CD3
Catalog Number: 16-0032
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: 17A2
Isotype: Rat IgG2b, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
|
|
Surface staining of mouse thymocytes with purified anti-mouse CD3 (17A2) followed by anti-rat IgG FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
|
| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-0032 |
Affinity Purified Rat anti-mouse CD3 (CD3e, epsilon subunit) |
N/A |
N/A |
FA
FC
IH/F
|
| 16-0032 |
Functional Grade* Purified Rat anti-mouse CD3 (CD3e, epsilon subunit) |
N/A |
N/A |
FA
FC
|
| 51-0032 |
Alexa Fluor® 647 anti-mouse CD3 (CD3e, epsilon subunit) |
633 |
668 |
FC
|
| 56-0032 |
Alexa Fluor® 700 anti-mouse CD3 (CD3e, epsilon subunit) (APC-Cy5.5 Equivalent) |
633 |
723 |
FC
|
| 57-0032 |
Pacific Blue® anti-mouse CD3 (CD3e, epsilon subunit) |
402 |
421 |
FC
|
| 27-0032 |
Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD3 (CD3e, epsilon subunit) (APC-Cy7 Equivalent) |
633 |
779 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits γ, δ and ε are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 17A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 17A2 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The 17A2 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Miescher, G. C., M. Schreyer, et al. Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex. Immunol Lett. 1989: 23(2): 113-8.
Zhang Y, McCormick LL, Desai SR, Wu C, Gilliam AC. Murine sclerodermatous graft-versus-host disease, a model for human scleroderma: cutaneous cytokines, chemokines, and immune cell activation.
J Immunol. 2002 Mar 15;168(6):3088-98.(17A2, IH/F,
PubMed)
Related Products
Cat.
16-4031 Functional Grade Purified Rat IgG2b Isotype Control
Mouse Cellular Antigens Chart